FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47182
Sequences flagged as poor quality0
Sequence length96-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5051.0703234284260947No Hit
CAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTATACACATCTGACGCTG3880.8223475054046034No Hit
GGTCTACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTGACGCTG3600.763002840066127No Hit
CAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTAT3590.7608833877326099No Hit
CAGTAGACCTGGCGTCAGTAGACCTGTCTCTTATACACATCTGACGCTGC3170.6718663897248951No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGCTGTCTCTTAT3120.66126912805731No Hit
GTCTACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTGACGCTGC2730.5786104870501463No Hit
CCAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTA2260.4789962273748463No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGGCTGTCTCTTA1810.38362087236658043No Hit
AGGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGCTGTCTCTTA1590.336992921029206No Hit
CTACTGACGCCAGGTCTACTGACTGTCTCTTATACACATCTGACGCTGCC1470.3115594930270018No Hit
GGTCTACTGACGCCAGGTCTACCTGTCTCTTATACACATCTGACGCTGCC1440.30520113602645077No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1430.30308168369293376No Hit
ACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTGACGCTGCCGAC1420.3009622313594167No Hit
CAGTAGACCTGGCGTCAGTCTGTCTCTTATACACATCTGACGCTGCCGAC1340.2840066126912806No Hit
GTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGGCGCTGTCTCTT1330.28188716035776357No Hit
CAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCTCTGTCTCTTA1310.27764825569072954No Hit
CGCCAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCTGTCTCTT1300.27552880335721247No Hit
GTAGACCTGGCGTCAGTAGACCCTGTCTCTTATACACATCTGACGCTGCC1200.2543342800220423No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG1080.2289008520198381No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1080.2289008520198381No Hit
TCAGTAGACCTGGCGTCAGTAGCTGTCTCTTATACACATCTGACGCTGCC1020.21618413801873598No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG940.1992285193505998No Hit
CTACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTGACGCTGCCG940.1992285193505998No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA910.19287016235004875No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG850.18015344834894664No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT840.1780339960154296No Hit
GAGGACAATTGAGGAAAGGTGATTCTTACAAGATGGTGAAGGTGCCATTG830.1759145436819126No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT820.17379509134839558No Hit
GGCCAGTAGTCGTTGCAGTGGGGGCAGCGCGGTTCAGCATTCGACTGGAA790.1674367343478445No Hit
AGTCAACAGTTACCACGCGCTGGAGGGGACCAATGAAGGAACGTAAGGCA760.16107837734729347No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT750.15895892501377645No Hit
CGCCAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCT750.15895892501377645No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG740.1568394726802594No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG720.15260056801322539No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC710.15048111567970834No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC680.1441227586791573No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT660.13988385401212325No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGGCGCTGTCTCT650.13776440167860624No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCCTGTCTCTTATACACATCTGA650.13776440167860624No Hit
GTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGCTGTCTCTTATA640.13564494934508925No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT630.1335254970115722No Hit
TCCTGTAACAGTGCGAGACCACTTCATGCCTTGCAGGTGCTGCTGCTTCT620.1314060446780552No Hit
CTCATATCCCGGGCTGCACACGCAGTCGTAGCTCCCCTCTGTGTTCCAGC620.1314060446780552No Hit
GAGGAGCTGTGCTCCGACCTGTTCCGAAGCACCCTGGAGCCCGTGGAGAA620.1314060446780552No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT610.12928659234453815No Hit
GTCTCCAGGTGTCGTCTGAGAATTCGAGGACGTGGTGCTGTGGGTGCTGC600.12716714001102114No Hit
GGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTATACACATCTGA600.12716714001102114No Hit
GGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAAT600.12716714001102114No Hit
GGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAA590.12504768767750413No Hit
GACTGCCCCAAGGCCCCCGCCGCCGCTCCAGCGCCGCGCAGCCACCGCCG580.12292823534398711No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC580.12292823534398711No Hit
GTTCATCTTCCTGCACACGGGGCTGGTGGTAGGTGTGAGCCTTCTTGCTC580.12292823534398711No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC570.1208087830104701No Hit
GGACGCGACCCCCGCGGCAGTCGAGATGGCCTTTTTAAAATTCTATATTT560.11868933067695309No Hit
CCGCCGGAGGCCAGGTTGCCCGCAAAGTACCTCCAGAACTGCGTGTGCTT550.11656987834343605No Hit
GGGCAGATTCCTGTCAAGAGACCTAAGGTTGTCATGGAAAGCATCACTTT540.11445042600991905No Hit
CAGTAGACCTGGCGTCAGTAGCTGTCTCTTATACACATCTGACGCTGCCG530.112330973676402No Hit
TTCCCCAGGCAGAGAATTCCCCAGCTCCTTTGAGTCCCTGGTGAGGAAGA510.10809206900936799No Hit
AAATAACCGTCCGCGACGCCGAGACAAACCGGACCCGCAACCACCATGAA510.10809206900936799No Hit
GACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTATACACA500.10597261667585095No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA500.10597261667585095No Hit
GATGTGGAATGGTGGGGGAGGGTCTAGGGCCTGGGCCCGCCCCTCACACA480.10173371200881691No Hit
GATTCAAGAACTTATGAGAGCCCAGGGGATCTGGAAAACCAACAGCAGTG480.10173371200881691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)