FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87418
Sequences flagged as poor quality0
Sequence length92-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT5120.5856917339678327No Hit
GGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT4350.49760918803907667No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA3630.4152462879498502No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTA2750.3145805211741289No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA2290.2619597794504564No Hit
GTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGCGCTCCCATCT2220.253952275275115No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC2150.2459447710997735No Hit
GAGATATTCCGCCAAGCCAGATTCGGGCGCTCCCATCTTGCGCAAGTTGG1870.21391475439840765No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1860.21277082523050175No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1700.19446795854400697No Hit
GTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT1670.1910361710402892No Hit
GGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGA1440.16472580017845295No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA1410.16129401267473517No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC1340.15328650849939374No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT1260.14413507515614632No Hit
GTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTAC1160.1326957834770871No Hit
AAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCT1160.1326957834770871No Hit
CATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAA1140.13040792514127525No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA1120.12812006680546342No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC1080.12354435013383971No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.1224004209659338No Hit
GCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTCATTG1070.1224004209659338No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA1060.12125649179802786No Hit
GTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCAT1050.12011256263012193No Hit
GATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA1030.1178247042943101No Hit
TAGCTATGTAGATTCAAATAAGAGAAATTGTGCAGCTGTTATTGGCAACT1030.1178247042943101No Hit
GTGCTACACCACGCATGGCCTAGAGCTGACCCGCGTCACCGTGGTGGACG1030.1178247042943101No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGAT1020.11668077512640418No Hit
GAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA980.11210505845478048No Hit
ACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGG920.10524148344734494No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA920.10524148344734494No Hit
CAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCT920.10524148344734494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)