FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87418
Sequences flagged as poor quality0
Sequence length91-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT4460.5101924088860418No Hit
GGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT4430.506760621382324No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA2590.29627765448763416No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC2420.2768308586332334No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA2390.2733990711295156No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTA2220.253952275275115No Hit
GGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGA1850.21162689606259577No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1700.19446795854400697No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT1520.17387723352170034No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1410.16129401267473517No Hit
GTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCAT1300.14871079182777003No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1300.14871079182777003No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA1220.13955935848452264No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA1180.13498364181289893No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.13040792514127525No Hit
GACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTA1100.12583220846965154No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC1100.12583220846965154No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA1090.12468827930174563No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA1080.12354435013383971No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG1070.1224004209659338No Hit
CATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAA1060.12125649179802786No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT1040.11896863346221602No Hit
GTCAAAGAGATATTCCGCCAAGCCAGATTCGGGCGCTCCCATCTTGCGCA970.11096112928687454No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT960.10981720011896863No Hit
AAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTA940.10752934178315679No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA940.10752934178315679No Hit
GGCGACGTCGGCCTCGGTGACTCCGGAAAACCTGGTGTTGTAGTCCACGA910.10409755427943901No Hit
TGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTT910.10409755427943901No Hit
GATATGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA890.10180969594362718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)