FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007661529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42685220
Sequences flagged as poor quality0
Sequence length87-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA4107700.9623237270418191No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG2749780.6441995613469955No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG2570220.6021334785201997No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA2098310.49157764678265686No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT1919660.4497247525021541No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG1834800.4298443348775056No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1711060.4008553780442036No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC1676840.39283855161107284No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1670430.39133686086190955No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT1479160.3465274397086392No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC1377660.32274871723748877No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA1341860.3143617392624426No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT1297140.30388504498746877No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA1267070.29684045203468556No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG1246380.2919933410206155No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC1219040.2855883137067116No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT1200960.2813526555561855No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA1190500.2789021586394541No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC1176070.27552159740537824No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG1147410.2688073295627854No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA1130240.2647848599585524No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT1042130.24414305466857145No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA1016090.23804258242080045No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT952020.22303270312300136No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG911640.213572754222656No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC874450.20486013659997537No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT859670.2013975797711714No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC832700.1950792335145514No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT827460.19385164232490779No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA827200.19379073131168117No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG813610.19060695950495277No Hit
GCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTA811310.1900681313110252No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTC787190.1844174634686198No Hit
ACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGC778290.18233243263124801No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG763720.17891907315928088No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAA752250.176231960383477No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA722560.16927639121925575No Hit
CGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCAC717490.16808862646133718No Hit
GGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAAT692010.16211934716513116No Hit
GGGCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAA688570.1613134476055178No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCCGAGTT673760.15784386258287997No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC655270.15351215244995808No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG648580.1519448652250123No Hit
CCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCA636410.1490937612597522No Hit
AATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCT634480.14864161412310864No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATC634140.14856196125965848No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC631050.14783805729477323No Hit
CGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAA602700.1411964141217967No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT591120.13848353130193541No Hit
TTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAA584270.1368787603765425No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCG575860.13490852337179005No Hit
AGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACG561140.13146002293065376No Hit
GGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAAT558750.13090011015522468No Hit
GCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGT534590.12524007138770749No Hit
AGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCC527920.12367746962531762No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA527250.12352050662969524No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG515510.1207701401093868No Hit
CAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGCAAACAGT486560.11398793305973356No Hit
GCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATG485370.11370914803765798No Hit
ATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGA482180.11296181675999326No Hit
CGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCC477560.11187947490958229No Hit
GACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACC476630.11166160090073332No Hit
CGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG469450.10997951984316819No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCG460740.10793900090007735No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA456980.10705813393956973No Hit
GCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAG456690.10699019473250927No Hit
TTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTT450970.10565015244152426No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT450440.10552598768379312No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG443430.10388373305795309No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)