FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007661531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38281357
Sequences flagged as poor quality0
Sequence length87-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA4484031.171335174978254No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA3625730.9471268220716419No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG2436870.6365683431755045No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG2386510.6234131146395882No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG1665920.43517788567421994No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT1613620.42151588304458487No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT1550240.40495952115804046No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1538430.40187446855658754No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC1524710.3982904785742052No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1430750.37374589411759884No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG1281800.3348366151179019No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA1259030.3288885501106975No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC1199910.3134450014402572No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA1189030.31060288693527766No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT1181350.30859668846117444No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG1111700.2904024536016317No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC1108900.28967102707461495No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA1082630.28280867890863953No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC1060060.2769128586533649No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA1032620.2697448786886003No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT1001120.26151633025966137No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT906770.23686986853679193No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA894070.2335523267892515No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT876190.22888164596673No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT867720.22666908072250416No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG851860.22252607189447332No Hit
GCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTA829730.2167451900934442No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC801500.20937084335855705No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC784120.2048307744158599No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA758650.19817740525760358No Hit
TTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAA751710.19636451236564054No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT748480.1955207596219747No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTC747690.19531439285185212No Hit
ACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGC744230.1944105586434671No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAA723890.18909726737220942No Hit
CGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCAC703370.1837369558242149No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG700990.18311524327625064No Hit
GCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATG691960.18075639272662147No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGG679980.1776269320860282No Hit
CCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCA676160.17662905732416956No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC664160.17349437220838332No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC662530.17306857748015567No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCCGAGTT648930.16951593434893125No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA648710.1694584651218085No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA627600.16394403155562118No Hit
CGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAA624150.16304280958483264No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG620460.16207889391172836No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATC610930.15958943148227478No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT602840.15747613126671556No Hit
GGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAAT600890.15696674493540028No Hit
GGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAAT563120.14710032353346303No Hit
AGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACG557330.14558783796509617No Hit
AGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCC547190.14293902904225678No Hit
GCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGT536000.1400159351717861No Hit
AATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCT534150.1395326712164357No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA508280.13277481255431986No Hit
GGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAA498140.13012600363148047No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG488530.12761564330125497No Hit
CGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCC486030.12696258390213283No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCG483980.12642707519485266No Hit
ATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGA470440.12289010548920719No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG456090.11914154453824613No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCG445330.11633077688442445No Hit
GACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACC428060.11181944255528872No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC424340.110847690169395No Hit
GCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAG422640.11040360977799195No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT418360.10928557208669484No Hit
GTCTAGACTTTCCTCTTTGCCTTTCGCCAAGTTTCTCTTAGTTTGTCCTT409900.10707561908006552No Hit
TTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTT409320.10692410929946919No Hit
GCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTC406710.10624231528678568No Hit
GGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCA406530.10619529501004889No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA396640.10361179202712172No Hit
TGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTG394780.10312591583417484No Hit
CTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGT384840.10052935166326522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)