FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007661547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33424688
Sequences flagged as poor quality0
Sequence length85-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2607720.7801778134772717No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG1905280.5700217755211358No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG1760620.5267423887397243No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1434220.4290900187310649No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT1366450.4088145863919508No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACCGTTTGCATTAATAAATTAAAG1238140.3704267935126276No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC1192490.3567692239939532No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC1186840.35507885668222244No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1183360.35403771009021834No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1167610.3493256242212343No Hit
GTTAGGTACCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT1047950.3135257388191627No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT989940.29617030381854276No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA916400.274168602561077No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA873710.2613966060057165No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG867570.2595596404669507No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG839370.25112276291105545No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC838900.2509821482851239No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT783200.23431781921195494No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA771130.23070671594600972No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA763940.22855561134931163No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG755090.22590786786102537No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA751350.22478893445467613No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT714880.21387783784249534No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT712640.21320767451890651No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC666220.1993197363577485No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCCGAGTT661690.19796445070781213No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC655030.19597191154035604No Hit
ACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGC634980.18997335143412558No Hit
GCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTA594900.17798221482276813No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG590800.1767555765965564No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG575170.17207939233419323No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC569260.1703112382081173No Hit
CGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCAC568600.17011377937170274No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG558730.1671608722271394No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT549530.1644084157195424No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA549140.16429173549802467No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT544720.16296935965415743No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTC515930.1543559658657098No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATC515370.1541884250348126No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCG499410.1494135113542421No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAA496280.14847707778154878No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA493650.14769023423644223No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG463660.13871782438178631No Hit
CCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCA458210.13708729308109024No Hit
AATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCT452730.13544778637873897No Hit
GGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAAT445890.13340139480135163No Hit
CGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAA441950.13222262538396767No Hit
GGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACCGTTTGCATTAAT437720.1309570937505834No Hit
GGGCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAA432760.1294731606769224No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC425290.12723828566477569No Hit
GGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCG420000.1256556231729074No Hit
CGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCC403780.1208029226779918No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT393330.11767649110142778No Hit
GCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGT388740.11630325464818102No Hit
TTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAA387740.11600407459300742No Hit
AGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACG382760.11451415791824296No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCG369380.11051112878002034No Hit
AGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCC368520.11025383393257104No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA366110.10953280999960269No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA365200.10926055614939473No Hit
CAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGCAAACGGT354520.10606531316014078No Hit
GCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTT341260.102098185628539No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)