FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91889
Sequences flagged as poor quality0
Sequence length92-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA7360.8009663833538291No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA7310.7955250356408274No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG3910.4255133911567217No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC3770.41027761756031733No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT3650.39721838304911355No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG3340.363482027228504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3060.3330104800356953No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT3030.3297456714078943No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT2550.2775087333630794No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA2420.2633612293092753No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT2350.2557433425110731No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA2320.25247853388327224No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG2300.2503019947980716No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG2070.22527179531826444No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA2050.2230952562330638No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA2010.21874217806266255No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT1980.21547736943486165No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA1980.21547736943486165No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC1840.20024159583845727No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC1820.19806505675325664No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG1750.19044716995505448No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT1750.19044716995505448No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG1660.18065274407165166No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA1590.17303485727344947No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC1570.17085831818824887No Hit
TTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAA1570.17085831818824887No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1560.16977004864564854No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA1490.16215216184744638No Hit
TCTCACAGGAGCGCATCCGGCTGCCCACTGGCCGCCCGCAGGCAGAAGGA1400.1523577359640436No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG1330.1447398491658414No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGG1310.14256331008064077No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT1310.14256331008064077No Hit
AGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACG1300.14147504053804047No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT1290.14038677099544014No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG1270.1382102319102395No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC1260.1371219623676392No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG1240.1349454232824386No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATC1230.13385715373983828No Hit
TTGTAGGCCTTTTTGTACAGCCTGTCATTCTGCATCTTCAACACATTTGC1230.13385715373983828No Hit
GCGGCGAGTAGGCCGCGGTGGAAGGAGAGCACCTCGCGGCCGACGAAACC1220.13276888419723798No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC1220.13276888419723798No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC1190.12950407556943705No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC1180.12841580602683672No Hit
GCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATG1150.1251509973990358No Hit
CGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCAC1130.12297445831383517No Hit
GTAAGTAGCCGTGCAGACACCCTGACTGTTCACTCACTACCACTCCATAG1100.11970964968603423No Hit
GCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGT1040.11318003243043237No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTC1010.10991522380263143No Hit
CCATTATCCTCCGAAGCTCTGGGTGCCAAGTCCCCACCGTCCTCCGCCGA1010.10991522380263143No Hit
GGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAA1000.10882695426003113No Hit
GGCTGTAAGTTGAAGGGTGACCCTGTCTCCACAGGTGACATTGGGGGCCA990.10773868471743082No Hit
CTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGT990.10773868471743082No Hit
ATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGA980.1066504151748305No Hit
GTGTCCCCTCTTTGGAATCCTTATTCAGCTCTGAATTCACAATCCGTCCC970.10556214563223018No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAA970.10556214563223018No Hit
CGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCC960.10447387608962988No Hit
GGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAAT950.10338560654702958No Hit
GTTCTTGCCATACTAGTTGCTATGGCACAGTGTCTTCCTTAAACGGAGAG950.10338560654702958No Hit
CCACCGTCCTGGGTCAGGTGTTCCGTCTCCTGGACTCTGGCTTCCAGGGG940.10229733700442926No Hit
GCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAAT930.10120906746182894No Hit
GTCCTTGCGCAGCTTGGCCAGCAGCCGGGATGCCCACTTCTGCTTGATCT920.10012079791922864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)