FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007680850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007680850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200046858
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACTTTGGCATAACTTCCTCTTCTAGTTTAGGACCTAGAGACTACTGC100907185.044177199723877No Hit
GCTCACTGGAGTATCTGTTTGCTCAGGTAGGAAGTTTGGGCCCAGGAGAA15852720.7924503368105887No Hit
CCAATAATCTCTTAAAACAGGCCTCTTTTTTTTATGCACAACAACTTCCT14964120.7480307438770171No Hit
GACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGAGACCGG14554000.7275295471024094No Hit
CTTTTGTACAGAGGTGGCAGAAACACTGTAACGCTGGAAAAAGAGAAAGA13652700.6824751029081396No Hit
GTATCGTCGTCCTCCCCGCAGTCAGGCTGCCCATCACCCACCATTCCAGC12821760.6409378346747141No Hit
TTGGGTAATCCGGTCCCCAGCCTTTCACAAAACTCATCCTGAGTATGCAT12646720.6321878847004936No Hit
CATATTCATGGAGAGAAGAAGGAGTTCGTGTGCAGGTGGCTGGACTGCTC12556910.6276984365333046No Hit
AGAACCATAAGCTCCTCGTCCAATTTCTCCAAGGTCTTTCAAGTCCTCTG12477020.6237048721854956No Hit
TGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTA12315790.615645260471924No Hit
CCCTTTCCTCAGAATTACTCAGCTGCGGAGGATCTCCTTTCACGACTTGT12182200.6089673250454151No Hit
CACACCTACCTCCCACTGCATTACAGCGAGATGTCATTTCCCAGAGCACC11016860.5507139732232136No Hit
TGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCAC10939610.5468523779563685No Hit
GTTGCCAAGCCAAAGTCTGAGATTTTGAGGTTATCTAAACCAAAAGAAAA10654850.5326177129960222No Hit
CATGTGTGACTTGATTAGCAGGTCAAAAGTGAACTGATGCAGCTCTCTCG9950920.4974294572524603No Hit
GTCTGGCTCCGGTAAGTAAGAGTGCCAACCAAAAATGCAAGTGCACCATC9380830.46893163400746835No Hit
TTTGGTGAATGGCTGGTGCATAAGTTTGACCTTGTTGAGCTGGCTGGCTT9092160.45450151484008816No Hit
ACAAATGTTTCAGAACTTCAAACTATGTGCCCTTCAGAAGTAACAAATAT9063820.45308484675125466No Hit
ACTTCTTCTTGCGTTCCTCAGTACTGGGTGGAGCAGCCCCCGGTTTGTCA8987360.4492627422321225No Hit
TGTCAGAAGAACCCCTGGCCCCCTCCAGCATAGATGCTGAAAGCAATGGA8898870.44483927860541556No Hit
CTGTGCCCCTAACTGTGTGGCCGAAGTCGTGACATTTGACAAAGAGGACA8398450.4198241394023794No Hit
CTCAACAGTCATCAACCACTACCGCATGCGGGGCCATAGCCCCTTTGCCA8379460.4188748618086269No Hit
CCCAGCCTCCAATTACGCGGACCCCTACCGACAGCTGCAGTATTGTCCCT8363590.4180815476741954No Hit
GCCTGTAGTAATCAAGTGTCTCATTTTTTCATTTCTTGTAGCAGAAACTT8362740.418039057629188No Hit
TACTTTCCCGTGGCCGACCTGTCTATCATCGCCGCCCTCTATGCCCGCTT8339340.4168693316842797No Hit
ATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGAGTGGGTCCCA8327400.41627247152264696No Hit
TGCCAAGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTTTGTGGGT8288890.4143474225423725No Hit
ATGGAAACCTTGCGCCCTCGCTCTAGAGAGCTGGTGTCCATTTCACTGTC8203440.41007592331192727No Hit
GCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATGTGTG7907520.39528338905477833No Hit
CTCTGTTTTCCTGCTCCTATCAGGTGTGTGTCCTGTATGCAGTGTGGGGC7608350.38032839286083664No Hit
GTACATGAACTTGTCTTCCCGTCGTGTGGGTCCTGAATTGGAGGAATATA7538100.3768167156116993No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTTAAATAGA7370510.3684391783848962No Hit
GGTTAGAATTTCTAAGAAGTTATCCTGTTTTTTTTCTCTCTTAGATCTGT7351260.3674769038362002No Hit
CACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATCACTGTGGAGGT7135890.35671092619710126No Hit
TGGGTGAAATGGCAACTGTTGCCCCAGTTCTCTACACCAATATTAATTTC7110760.3554547205135309No Hit
CACATGCCATTTTTCTTCACCAGAATGTTCTTTGACTTTAAGTCTCGATG7083700.3541020374336497No Hit
CTTCCGTGGCAGATTGTGCAGGTATAGACCTTGATCCAAGGGGAGGGCAG7038110.3518230713726081No Hit
CCATGCCTACTAGCCTCCCTAACCTTGCCAAGGAGGCAAAGGATGCCAAG6979930.3489147527625753No Hit
TATGCTCAGTGGTGTCCCCCCCACCAATCCGCCAGTGCAGCAGGCCACTG6972920.3485643348619852No Hit
ATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTA6939450.3468912268544603No Hit
CCTTTCAGTTTCTCTTGTAGTCTCATTAAGACACGTTCAGCTACTTTGTT6853890.34261422891230814No Hit
GCAGGGGCATACTAATAGAGTCTATTCATTACAGGTAAGATCTCTTATCT6808040.34032226589632314No Hit
GTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGAGACAACATCAT6748210.33733146661068775No Hit
TCCAAGGAAGGCAGTGACCTGTCAGTGGTGGAGCGTGCAGAAGTCTGGCT6704540.3351484780630746No Hit
GGTTATCCATGAGCTTGAGATTGATTTTGTAGTCACCCACCTCTAAGGCC6650860.3324651067501395No Hit
GCCCAGCACTTGAGGGTACCTGGCACTGCAGCAGGCGAGAGTGGTCCTGA6604900.3301676450224477No Hit
AGGATGTTGTAATAGGGTGGCTTAGGTGTAACTGGATTTGAAGTGGCTGT6589180.32938182913125286No Hit
AAACCTCCAGACACACCTAGGCCTGTGATGACAGATCAATTTCCCAAATC6509990.32542325658521465No Hit
GGGCTATCTCACTTCTCAAGAACCAAAACAAAAAGTGACCAATTCTTTTG6263390.31309614470425723No Hit
ACGTCTTTGGTGTGCTGCAGGCCAAGCAAGGCCAGGAAGCCCTTCTGGTC6189250.309390013013851No Hit
CCAATTGCCTTCTGGTCATATCTGTTTGTAAGGAAAACTAAAACCACAAA6154750.3076654170694348No Hit
CATCTTGCTGAGGGGAAGGCTGGGCAGCTTGTTGCTGGCTGGCAGGCTGG6138930.30687460234941555No Hit
GGACTTCTGGTCGAACATGCCGGCCTGGATCACCTTCTGGTCCACGTTGA6072430.3035503811811931No Hit
CCTATACACTGGAACATTTGCTTCATAAAGATCTTCAAGATTGGGCCACC5939020.29688144364656804No Hit
ATTTGGCCAGTGCTATCCTTGACCTGCCAGTCCTGGAGCACCTCTTTGCC5901520.29500688283742No Hit
CCAGCTTGCCAGATTTTTCCCCATTACACCCCCAGTGTGGCATATCCTTG5898950.2948784129366331No Hit
CTTGAATAAAAGACAATGTACTATGCTCAATTTTTTAAAGTAACTATCTG5892240.2945429915225162No Hit
GGGAGACCTCTTAGATCAGTTCTTTTGTACTACTTGTGGTCAGCACTATC5829230.2913932294802651No Hit
ATCCTTATCTCCCTTGCTTGGAGAGCCAGCCCTGTCTGAGCCTGGGGAAC5514690.27566991329601387No Hit
CAGATGCAATGATCCTTGTGGATGGACAGCCACGCCAGGCCAAGGGTGAG5509860.2754284698637956No Hit
ATTCCTCACAGCATTGCCCATGACGCCGAGCCCTGCTCTGACCCTAGGAC5504610.2751660313505149No Hit
GGAAGTCATTGAATCCTTTGCCATTAACATGGAAACAGGCTGGATTACAA5481850.27402829791008265No Hit
ACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGA5474810.27367638036084524No Hit
GCCAAGAATCAGCAAGCCATGGATAGCTGGGATCACCAGCATTGAGAAGA5330540.26646457001589097No Hit
AAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGAT5316530.26576423409759325No Hit
GTGCAAAAGAAATGCCAAGAAGGCTGAGTCGGGGAAGAGCGCCAGCACCG5303410.26510838775583273No Hit
AGGATGAGGACAGTGAAGGCCCAGGGAGTGGAGAGAGTCGCCTACAGCAT5187290.2593037477249455No Hit
CATCTTTGTGATCATCCTGGCTTCGGTGCTGCGCATCCGGTGCCGCCCCC5130490.2564644129526893No Hit
AATGACTGGACAAAAGGTGACTTATACTTACAATTTCATTGTAAGTGGGG5127800.2563299444573131No Hit
CTTTGAAAACGGTCCTGCAAACCGCCACAAGCCATTAAAACCAAAACCTG5123330.25610649680886266No Hit
AGGTACTTTGTTGTTCAGTTTACTCTCAAAATTGCTCATAATGAAAGACA5095570.2547188219272107No Hit
TCAACAAGATGTTTTGCCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGG5081220.25400148999091No Hit
GGATTCATCAGCATCGTCAAGCTGGAGAGTCCTCGACGGGCCCCCCGCCC4894600.24467267563882455No Hit
GTGATGTAGAATTTCAAGTTTTAAATCACAATGTGAAAAGTGCATAATTT4885370.24421128373833295No Hit
ACATTTTTGTCTTACCTAGTTCATTCATTGCATGTCTATGCTCTTCATCA4870870.2434864535587957No Hit
CTACCGCCTCCTGCATGATGGAGATGTTTATATCTTTACTGACTACTGCG4864600.24317302699150617No Hit
CATGGTGCCGCGCTTTACCGACGAGCAAGGGTCATCTGCAAACTCGGAGG4858640.24287509679357225No Hit
CTAATACATTCTTGCTGCCTATGAATAATATAAATATATTTTCTAAGGAA4794790.23968334458919618No Hit
CTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGCTC4722040.23604669661944902No Hit
CAGCCTGAATTATGATCCTTTCCTAGGTACCCCATGGCCTTAGGGAACCT4681840.23403716743204236No Hit
CGCTCAGGCATTCCTCCGATCGCACACATTTGTCGTGGCACCAGCCACAC4618800.23088590574114393No Hit
TTAAAAATAGGGTTTGGCTGCTTTCTTGAAGGCTATTGGGTATCTTATTC4574180.22865542831969898No Hit
CCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGA4486630.22427895368394138No Hit
TCCTGCAAGCAGCACAAAACCGAGGACCAGCTTTACCTGGCCCGTTGGTA4456760.22278580351409466No Hit
TGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATGCCAGT4376210.21875924689604473No Hit
GCAAGTGAGGTTAGAAAGAATTCCAGCTGCACAGGTGACCACATTTATAT4347970.21734757763603565No Hit
GGCATCTTACCAGGCAGTCGCTCTCCCGGGGGCCTGTGCAGCCTGCAGCA4344330.21716562026682767No Hit
GCAAATGAAAACCAAAACTTAAAAGAACAAAATCAAACAGAGAAATCTTT4294730.2146862011699279No Hit
GCCTTTGACGAAGCTCATGCGGATGGTGCACATTCGGGTCAACTGGTAGA4285540.214226808800966No Hit
GGATGATTGGAAATGGGAGGCTGGAATGCAAGCTCATTGTGAACAGGCCC4281190.21400935974710483No Hit
GGTCTTTCGAATGTATGCAATGTCATCAAAAGATTGTAGTTCTGGCATTC4260510.21297560194622No Hit
ATTGAACTCCAGCTGCCTGAGGTGATGGGGACTTGGAAGGGACACAGGGG4237830.2118418675688473No Hit
CAGATGGAGGTGATGCTGCAGTTGCTCATGCAGGATTTCTGGTTGTCACA4180970.20899953349929645No Hit
GCAATCACTACTCCGCATTACTACATCCAAATCCATAAGAAGTTGTTTTT4148190.20736091741065987No Hit
TGAGGGTTTCCAAGACCAGTCTCTGCGGGGAAAGGACGGCATTGGGGCCC4106750.20528940274583068No Hit
CCTCCAGCGACTATACTGAATACAGTGAAGAAGAGTCTGAGTTCAGTGAG4054520.2026785144508493No Hit
GCTTCTCAATACCCTGTGTCTCTCGAAGCTTTTTGGCAGCCTCCAAGTTC4050280.20246656410869499No Hit
AGATTTTGAGGGTGCCACCGGTGGTGAGCAGCAGGTTCCCCGGCTTGATG3935490.19672840850117226No Hit
TCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTGTCTGGCTCAGCCTGGA3881970.1940530353143562No Hit
TGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGC3834430.19167659209123894No Hit
GGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTTCACAT3797590.18983502355233192No Hit
GCGATACACTCTACAGCAGACTGCCAGTCCTTGTAATTTTTATCAAAGTT3774640.18868779233713334No Hit
CTCTACTGTGTGCCTCTGCAGGCTCGGATGGCGGGTGAGCGAGGAGCCAG3672580.1835859876389561No Hit
GGTAGCATTAGACTCAGATGGGGCTAACAGAGCTGGGGTGCTGTATGTCT3669740.18344402090034326No Hit
ACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCATAAAAAA3625490.18123203914554858No Hit
CTTGTGCAGTAGCCGCCTGCTGCTGCATCTGTCGATGACACTGACGCAAA3622320.18107357627181528No Hit
CCAGGATAGTCTTTCTCAGGCTCAGACTTCTCAGCCACCCTCACCGCAAG3508240.17537091234894575No Hit
TCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTT3494380.17467807467388466No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTCCCCCAGAACGGAG3474350.1736768092603584No Hit
TCCTTCCCTGTCTAATTGCTTTTTTTTTTTTTGTAGTAATAAAAGAGCAG3455450.1727320306125478No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3448300.17237461435160356No Hit
GCCCAACGCACCGAATAGTTACGGTCGGAGGCCGATCCAGGTGGGTAGAG3443540.17213667009956238No Hit
GCTTCCGAGTCTCCCTTCAGCCCTCCACATCCCTACATTAATCCCTACAT3441600.1720396928203691No Hit
AGGCACAAGGGGTCTGAGTACCTGTGAGATGGCCCACTGGGCCGCAGCAA3419390.17092945293847103No Hit
GGAAGCATGAGTACTGTGGTAAGTACTAAAACTATTAAGACAAGCCACTA3415840.17075199451520504No Hit
TCAAAGATTCCAGAAGTCAGGACACGGCAGCGAAGCAGGTCCTGAAACAA3411440.17053204604693167No Hit
ATCAGTGCTTTGGCCATTGCTGCCTTGGCTGAAGCAGCAACTCCTTATGG3378290.1688749342916448No Hit
AGTATATATCCGAGCCGCTGTCTCTTATACACATCTGACGCTGCCGACGA3349310.1674262736983352No Hit
TCAAAGGCATAGAGACACTGGATATACTGCTTTTTCAAGGAGCTGGCAGC3277070.16381511975559246No Hit
GGGGTAGTTAGGGCAGGAAATGCATTATCTCAGAGGATGGCTCCACAAGA3218360.16088030735279032No Hit
CCGTGTCATAGAGCAGCGCATCCCCTACCGTGGCACCCGCTACCCAGAGG3216630.1607938276141283No Hit
TGCTGCCGGATGTGGTACTGCTGCTGCTGCTGCTGCTGTTGCTGTTGCTG3203220.16012348466877696No Hit
CCACATGGGTTAATTTGCTTTTATAAAGGCTGCCTACTTTTTTCTCAACT3102290.15507816673631533No Hit
AGCTAAAGGATTAATGGCACCTAAAAATAAAAAGCACTCATTAACACTAA3074260.15367699501683751No Hit
TGTGCCTACCACGTAACCAAAAATGAAGGAGAACTACTGTTTACAAGCCG3069630.15344554924226803No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTTCCCCCAGAACGGA2809900.1404620911366676No Hit
GCATGTATAGCACCTCGAGTGTTGAGATTACAAGCAGCACATCAGGTAAA2802730.1401036751099585No Hit
CTGCTGGACCCTGGTGTTCACCTGTTGAGCTGTCTTGGTCTTGTGCTTCT2785430.1392388777233382No Hit
GTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCAT2734670.13670147221207543No Hit
GTTATAGCTGTTATTAAAGAATCCAATTCATCTTTTTTCTCCTTCAGCTT2650340.13248595986446335No Hit
GCAAATGCTATCGATTTCTTGATCACATAGACTTCCATTTTCTACTTTTT2635400.1317391348380988No Hit
GCACTTTAGGATAACAAAGTCATACGAATTACATACCTTGAGAAAAGCTC2622470.13109278627110454No Hit
ATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTA2556830.12781155503077185No Hit
ACTGAGTTGCGATAATGTTTTCTTAATCCGCAATGCTGTAAGCCTAGCTG2542090.12707472766205605No Hit
CTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCT2504000.1251706737628441No Hit
GGAAGATAAACTAAAACGAGAAGAAGAAAAAAGAGGTTGGTTTCTTTTCA2464260.12318413918802965No Hit
GTACAGATGTATTTGGACTCATTCAATATTGCCCCGTTATAGAACCACTG2435200.12173147953166052No Hit
GTCTGTAAGGGCCTCGTGGCTGGGCCTCTGTGCTCCCCATCCCCCCACCC2432290.12158601361287064No Hit
CCACAGATTTTGCATCCTTAGCAACCCCTGCTTGTTCTGACTCCTGTTTT2400760.12000988288453898No Hit
CCTCCAGCGACTATACTGAATACAGTGAAGAAGCGTCTGAGTTCAGTGAG2335530.1167491468423863No Hit
GCCAAAGTCCTCGGACACTTCCACTCTCTTTTCAGGAGGCACAAAGATGG2282800.11411326440328294No Hit
ACCAATTCCAGGTGATACAACTCGTTCGTAGTGATATGGATTCACACAGA2237250.11183629787377114No Hit
TCCTGAAGGCTTGCTCTCTTTGGTTAAGGTAATGCCTTGTTGTCCACCTG2212710.1106095852802647No Hit
ACAGTAACTGTGTATCTGTTTAAGTAATCAAAAAGGGATCCATGCTCATG2195220.10973529011887805No Hit
GTCACAGAGCTTAATATTTCCACTTCTGTCCAGAAGAATATTGGAAGGTT2159900.10796970377810182No Hit
TCTTGGTGGATGACGGGCCGGAGCCGAGCAGCTGAAGGCACCCGCTGGGT2131210.10653553978838298No Hit
GGATTGACTTCAACTGGGAATACCCTTCTTCGAAGGTAACGCTAATGATT2115750.10576272085213155No Hit
TTCTCTGCGTTCAGCTGCACGGTCCCGGTACTTCATTTGCTGAAAGTTTG2038570.1019046247654637No Hit
ATCCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGCTGCATCC2003060.10012954064992113No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)