FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007680855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007680855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142385161
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGACAGTCAGTACGTACGAGTCCCAACGGAAGTACTCGCAGTAGTC8242470.578885464054783No Hit
ATTAGCGGTGTCAGTACGTACGAGTCTTACACCTTGTACTCGCAGTAGTC4718900.3314179628592055No Hit
TGTGTCTACGTCAGTACGTACGAGTCTCGCATTCGGTACTCGCAGTAGTC3891050.2732763704217745No Hit
TATGAGACCGTCAGTACGTACGAGTCGGAACCTAGGTACTCGCAGTAGTC3651550.2564557973846727No Hit
AGTCGCGACGTCAGTACGTACGAGTCTATAATCGCGTACTCGCAGTAGTC3431600.24100826068525497No Hit
TCCAAGTAAGTCAGTACGTACGAGTCGATTCTCGGGTACTCGCAGTAGTC3386220.2378211308129223No Hit
ACACACCGTGTCAGTACGTACGAGTCGACATATGTGTACTCGCAGTAGTC3325630.2335657716466676No Hit
GATACTGTGGTCAGTACGTACGAGTCGTCATTCTAGTACTCGCAGTAGTC3305730.2321681540957769No Hit
CTTGTAACGGTCAGTACGTACGAGTCGATCGTTGAGTACTCGCAGTAGTC3198360.224627340204363No Hit
TTCGTGATGGTCAGTACGTACGAGTCGAATAAGAGGTACTCGCAGTAGTC3196720.22451215966248056No Hit
CGAGTAGCAGTCAGTACGTACGAGTCATTCGAAGAGTACTCGCAGTAGTC3093430.21725789248501814No Hit
CAGAGCACCGTCAGTACGTACGAGTCAGTTATCGCGTACTCGCAGTAGTC3090030.2170191035567253No Hit
CCAGTTACGGTCAGTACGTACGAGTCCGTGTATTCGTACTCGCAGTAGTC3070660.21565870898583314No Hit
TACAGTTAGGTCAGTACGTACGAGTCTACTTACCAGTACTCGCAGTAGTC3027930.21265769401349346No Hit
ACGCTCTTAGTCAGTACGTACGAGTCTGGTGCCTGGTACTCGCAGTAGTC3021610.2122138275350196No Hit
AGACCACGCGTCAGTACGTACGAGTCCAGCGTAAGGTACTCGCAGTAGTC2977810.20913766428230537No Hit
CCTAGAGTAGTCAGTACGTACGAGTCATCCACAGTGTACTCGCAGTAGTC2944720.2068136861537137No Hit
CGGTCGACAGTCAGTACGTACGAGTCCTCCTGACGGTACTCGCAGTAGTC2902010.20381407582212868No Hit
CCATTCTGCGTCAGTACGTACGAGTCTAATCTGGAGTACTCGCAGTAGTC2778350.19512918203604096No Hit
TATGAGACCGTCAGTACGTACGAGTCATCTCGAACGTACTCGCAGTAGTC2703140.18984703047812684No Hit
AGATGAAGAGTCAGTACGTACGAGTCAATCAGGAGGTACTCGCAGTAGTC2615530.18369400165232105No Hit
AATCCGCTGGTCAGTACGTACGAGTCCTGAGTCCAGTACTCGCAGTAGTC2579670.18117548077920845No Hit
CAAGCTGCAGTCAGTACGTACGAGTCCTATTCCGTGTACTCGCAGTAGTC2574660.1808236182701651No Hit
CCATAGCCAGTCAGTACGTACGAGTCCGAGCTTACGTACTCGCAGTAGTC2551250.17917948626683086No Hit
ACTGGACCAGTCAGTACGTACGAGTCTGGAAGATTGTACTCGCAGTAGTC2546830.1788690606600501No Hit
TCGACGTTGGTCAGTACGTACGAGTCTGCTCACACGTACTCGCAGTAGTC2472900.17367680611043448No Hit
AACGATGACGTCAGTACGTACGAGTCAACTTAGCTGTACTCGCAGTAGTC2464650.17309739179913558No Hit
ACTCTCACAGTCAGTACGTACGAGTCAGTCCGTGTGTACTCGCAGTAGTC2459320.17272305503801763No Hit
AATGCTATCGTCAGTACGTACGAGTCACGCACACTGTACTCGCAGTAGTC2459260.17271884111575364No Hit
GAACGCTGTGTCAGTACGTACGAGTCGCATGTGGTGTACTCGCAGTAGTC2298070.16139813895353886No Hit
CTGGACACTGTCAGTACGTACGAGTCCATCCAGGAGTACTCGCAGTAGTC2281780.160254059058865No Hit
TGCCAGACGGTCAGTACGTACGAGTCGAGTCAAGGGTACTCGCAGTAGTC2212360.15537855099942613No Hit
GCCGTGAACGTCAGTACGTACGAGTCTTGACCTGAGTACTCGCAGTAGTC2183480.15335024974969125No Hit
CAGCGTAAGGTCAGTACGTACGAGTCCGTGGTACCGTACTCGCAGTAGTC2173200.15262826440179394No Hit
TCACTACGTGTCAGTACGTACGAGTCCACTACAGGGTACTCGCAGTAGTC2171170.15248569336519555No Hit
GCAGCGATAGTCAGTACGTACGAGTCAATACGTCTGTACTCGCAGTAGTC2115280.1485604247762869No Hit
TCAGGTGCAGTCAGTACGTACGAGTCTCATCAGAGGTACTCGCAGTAGTC2102270.14764670596537796No Hit
TCGAATAGAGTCAGTACGTACGAGTCAAGTTGCCAGTACTCGCAGTAGTC2091420.1468846883559727No Hit
TTAACGTTCGTCAGTACGTACGAGTCCGTGTGTGGGTACTCGCAGTAGTC2070670.14542737357300878No Hit
TCCTCGTCGGTCAGTACGTACGAGTCCATATGCGAGTACTCGCAGTAGTC2017400.1416861129229611No Hit
CTTACATGCGTCAGTACGTACGAGTCCATGTCCTGGTACTCGCAGTAGTC1975050.13871178612496002No Hit
TGCAACTCTGTCAGTACGTACGAGTCCGTTACACCGTACTCGCAGTAGTC1966180.13808882795026653No Hit
GTACAATGCGTCAGTACGTACGAGTCACAGGAACTGTACTCGCAGTAGTC1905970.13386015695835046No Hit
ATTCATGTGGTCAGTACGTACGAGTCGCATGTAAGGTACTCGCAGTAGTC1888790.13265357055009405No Hit
CCATAGCCAGTCAGTACGTACGAGTCGTATTACTGGTACTCGCAGTAGTC1885510.13242320946632916No Hit
TGCCTTCAGGTCAGTACGTACGAGTCAAGGTGCTGGTACTCGCAGTAGTC1862180.13078469602601356No Hit
GATCCGCACGTCAGTACGTACGAGTCAGATCTTCAGTACTCGCAGTAGTC1860860.13069198973620572No Hit
GGATCGTTCGTCAGTACGTACGAGTCGATTAGTGTGTACTCGCAGTAGTC1822170.12797471219630815No Hit
TAGAGCGTCGTCAGTACGTACGAGTCGTCGCAGGTGTACTCGCAGTAGTC1821890.12795504722574286No Hit
GTCGTTAGAGTCAGTACGTACGAGTCCGGTCGACAGTACTCGCAGTAGTC1819950.12781879707254043No Hit
ACTCCAACTGTCAGTACGTACGAGTCTCTACATCCGTACTCGCAGTAGTC1791080.1257911981431829No Hit
AATGAGTCGGTCAGTACGTACGAGTCATTGATCACGTACTCGCAGTAGTC1759330.12356133094515376No Hit
ACCACGGATGTCAGTACGTACGAGTCAAGGTCTGAGTACTCGCAGTAGTC1756560.1233667882006328No Hit
GCAACACCTGTCAGTACGTACGAGTCCATGGTCGAGTACTCGCAGTAGTC1746250.12264269589160348No Hit
TGTCACATAGTCAGTACGTACGAGTCCAATCTCCGGTACTCGCAGTAGTC1724530.12111725603203834No Hit
ACTCCAACTGTCAGTACGTACGAGTCGAACTGCAAGTACTCGCAGTAGTC1712760.12029062494791855No Hit
GTGCGAGTAGTCAGTACGTACGAGTCTCACCGAGGGTACTCGCAGTAGTC1701880.11952650037738131No Hit
ATGCTCACCGTCAGTACGTACGAGTCGATAGTTCTGTACTCGCAGTAGTC1699710.11937409685550027No Hit
GGATACCTAGTCAGTACGTACGAGTCCCGCTCATGGTACTCGCAGTAGTC1683420.11823001696082641No Hit
CAGCCGCAAGTCAGTACGTACGAGTCTCCAAGTAAGTACTCGCAGTAGTC1666090.11701289574691004No Hit
ATGGAGAGTGTCAGTACGTACGAGTCGTGACGTAAGTACTCGCAGTAGTC1618130.11364456721722567No Hit
TCACACTGCGTCAGTACGTACGAGTCTGTCACATAGTACTCGCAGTAGTC1612790.11326952813573038No Hit
ACAGCGTATGTCAGTACGTACGAGTCCATCCAGGAGTACTCGCAGTAGTC1583530.1112145387116569No Hit
GACGTCAATGTCAGTACGTACGAGTCTCAGGACTGGTACTCGCAGTAGTC1555190.10922416276229797No Hit
CATCTCACGGTCAGTACGTACGAGTCTACTTCCAGGTACTCGCAGTAGTC1544510.10847408459930737No Hit
ACAACGAGCTCAGTACGTACGAGTCCGAGCCGTTGTACTCGCAGTAGTCG1513240.10627792877939016No Hit
GGTTAGATGGTCAGTACGTACGAGTCGAGGTCACGGTACTCGCAGTAGTC1511820.10617819928580899No Hit
GATTAGACATCAGTACGTACGAGTCGTGGTTACCGTACTCGCAGTAGTCG1503130.10556788287790748No Hit
CTCCGCATTGTCAGTACGTACGAGTCGATACTGTGGTACTCGCAGTAGTC1496640.105112076953019No Hit
AGAAGTAACTCAGTACGTACGAGTCAGCGTAGAAGTACTCGCAGTAGTCG1449980.1018350500723878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)