FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007680860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007680860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300825555
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTGGAGTATCTGTTTGCTCAGGTAGGAAGTTTGGGCCCAGGAGAA52486241.7447400703706837No Hit
GACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGAGACCGG44992841.4956455411509173No Hit
CCAATAATCTCTTAAAACAGGCCTCTTTTTTTTATGCACAACAACTTCCT42694871.4192567516413293No Hit
TGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCAC41576161.3820687540990326No Hit
CCCTTTCCTCAGAATTACTCAGCTGCGGAGGATCTCCTTTCACGACTTGT41472441.3786209087190082No Hit
CTTTTGTACAGAGGTGGCAGAAACACTGTAACGCTGGAAAAAGAGAAAGA39838721.3243130225422504No Hit
TTGGGTAATCCGGTCCCCAGCCTTTCACAAAACTCATCCTGAGTATGCAT39234711.3042346086588288No Hit
TGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTA38538631.2810956170262864No Hit
GTCTGGCTCCGGTAAGTAAGAGTGCCAACCAAAAATGCAAGTGCACCATC36185341.2028678880024006No Hit
GTTGCCAAGCCAAAGTCTGAGATTTTGAGGTTATCTAAACCAAAAGAAAA34899831.1601351487575582No Hit
AGGATGTTGTAATAGGGTGGCTTAGGTGTAACTGGATTTGAAGTGGCTGT34775221.1559928809904465No Hit
ACAAATGTTTCAGAACTTCAAACTATGTGCCCTTCAGAAGTAACAAATAT34012631.1306429734667989No Hit
CATATTCATGGAGAGAAGAAGGAGTTCGTGTGCAGGTGGCTGGACTGCTC33341951.1083483249951953No Hit
GTATCGTCGTCCTCCCCGCAGTCAGGCTGCCCATCACCCACCATTCCAGC32960881.0956808506511357No Hit
CCCAGCCTCCAATTACGCGGACCCCTACCGACAGCTGCAGTATTGTCCCT31574381.0495910162951416No Hit
AGAACCATAAGCTCCTCGTCCAATTTCTCCAAGGTCTTTCAAGTCCTCTG30943341.028614075024311No Hit
GCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATGTGTG28768290.9563113745439613No Hit
CTTCCGTGGCAGATTGTGCAGGTATAGACCTTGATCCAAGGGGAGGGCAG28422420.9448140135567937No Hit
CCTTTCAGTTTCTCTTGTAGTCTCATTAAGACACGTTCAGCTACTTTGTT27474510.9133037251439626No Hit
CTGTGCCCCTAACTGTGTGGCCGAAGTCGTGACATTTGACAAAGAGGACA27350530.9091823997465908No Hit
TGGGTGAAATGGCAACTGTTGCCCCAGTTCTCTACACCAATATTAATTTC26557940.8828352365210462No Hit
CTCTGTTTTCCTGCTCCTATCAGGTGTGTGTCCTGTATGCAGTGTGGGGC26360490.8762716319097292No Hit
TTTGGTGAATGGCTGGTGCATAAGTTTGACCTTGTTGAGCTGGCTGGCTT26224400.8717477476273583No Hit
TACTTTCCCGTGGCCGACCTGTCTATCATCGCCGCCCTCTATGCCCGCTT24919480.8283697839433887No Hit
TGTCAGAAGAACCCCTGGCCCCCTCCAGCATAGATGCTGAAAGCAATGGA24472590.8135143305893676No Hit
ATGGAAACCTTGCGCCCTCGCTCTAGAGAGCTGGTGTCCATTTCACTGTC24346580.809325524222834No Hit
GGTTAGAATTTCTAAGAAGTTATCCTGTTTTTTTTCTCTCTTAGATCTGT23633810.7856317260014696No Hit
GTACATGAACTTGTCTTCCCGTCGTGTGGGTCCTGAATTGGAGGAATATA23511210.7815562743663849No Hit
CCTCCAGCGACTATACTGAATACAGTGAAGAAGAGTCTGAGTTCAGTGAG23118750.7685101752741718No Hit
CCAATTGCCTTCTGGTCATATCTGTTTGTAAGGAAAACTAAAACCACAAA23047190.7661313880065808No Hit
GGGCTATCTCACTTCTCAAGAACCAAAACAAAAAGTGACCAATTCTTTTG22405760.7448090638443267No Hit
GCCTGTAGTAATCAAGTGTCTCATTTTTTCATTTCTTGTAGCAGAAACTT21459560.7133556190065036No Hit
ATCCTTATCTCCCTTGCTTGGAGAGCCAGCCCTGTCTGAGCCTGGGGAAC20989440.6977279573206472No Hit
GGGAGACCTCTTAGATCAGTTCTTTTGTACTACTTGTGGTCAGCACTATC20824300.6922383971002729No Hit
CTCAACAGTCATCAACCACTACCGCATGCGGGGCCATAGCCCCTTTGCCA20809890.6917593819447951No Hit
CCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGA20465280.6803039056971074No Hit
CATCTTTGTGATCATCCTGGCTTCGGTGCTGCGCATCCGGTGCCGCCCCC20236290.6726918529245296No Hit
TATGCTCAGTGGTGTCCCCCCCACCAATCCGCCAGTGCAGCAGGCCACTG20233140.6725871410758305No Hit
GTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGAGACAACATCAT20050310.6665095324099045No Hit
TCCAAGGAAGGCAGTGACCTGTCAGTGGTGGAGCGTGCAGAAGTCTGGCT19724310.6556726871159599No Hit
GCCAAGAATCAGCAAGCCATGGATAGCTGGGATCACCAGCATTGAGAAGA19518380.6488271915595735No Hit
CTTTGAAAACGGTCCTGCAAACCGCCACAAGCCATTAAAACCAAAACCTG19333220.6426721293674668No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTTAAATAGA19175400.6374258995383554No Hit
GGAAGTCATTGAATCCTTTGCCATTAACATGGAAACAGGCTGGATTACAA19003750.6317199348306696No Hit
TCCTGCAAGCAGCACAAAACCGAGGACCAGCTTTACCTGGCCCGTTGGTA18694140.6214279235685279No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTTCCCCCAGAACGGA18361070.6103560583475031No Hit
GCAGGGGCATACTAATAGAGTCTATTCATTACAGGTAAGATCTCTTATCT18104930.6018414891647087No Hit
ACTTCTTCTTGCGTTCCTCAGTACTGGGTGGAGCAGCCCCCGGTTTGTCA18036050.5995517900731538No Hit
GCCCAGCACTTGAGGGTACCTGGCACTGCAGCAGGCGAGAGTGGTCCTGA17767230.5906157141470245No Hit
CACATGCCATTTTTCTTCACCAGAATGTTCTTTGACTTTAAGTCTCGATG17626600.5859409118351No Hit
CACACCTACCTCCCACTGCATTACAGCGAGATGTCATTTCCCAGAGCACC17385070.5779120061791293No Hit
CCAGGATAGTCTTTCTCAGGCTCAGACTTCTCAGCCACCCTCACCGCAAG16850320.5601359232928199No Hit
CAGATGCAATGATCCTTGTGGATGGACAGCCACGCCAGGCCAAGGGTGAG16813830.5589229279407463No Hit
CGCTCAGGCATTCCTCCGATCGCACACATTTGTCGTGGCACCAGCCACAC16361300.5438799905147687No Hit
ACGTCTTTGGTGTGCTGCAGGCCAAGCAAGGCCAGGAAGCCCTTCTGGTC16216160.5390552674289922No Hit
ATTCCTCACAGCATTGCCCATGACGCCGAGCCCTGCTCTGACCCTAGGAC16206260.5387261730473663No Hit
GGATTCATCAGCATCGTCAAGCTGGAGAGTCCTCGACGGGCCCCCCGCCC15999360.5318484328899518No Hit
GCTTCTCAATACCCTGTGTCTCTCGAAGCTTTTTGGCAGCCTCCAAGTTC15933540.5296604538799904No Hit
CTCTACTGTGTGCCTCTGCAGGCTCGGATGGCGGGTGAGCGAGGAGCCAG15895030.5283803099773223No Hit
ACATTTTTGTCTTACCTAGTTCATTCATTGCATGTCTATGCTCTTCATCA15854590.5270360092911655No Hit
GCGATACACTCTACAGCAGACTGCCAGTCCTTGTAATTTTTATCAAAGTT15690110.521568388696233No Hit
TCAACAAGATGTTTTGCCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGG15652160.5203068602333336No Hit
CCATGCCTACTAGCCTCCCTAACCTTGCCAAGGAGGCAAAGGATGCCAAG15555750.5171020128260048No Hit
GGACTTCTGGTCGAACATGCCGGCCTGGATCACCTTCTGGTCCACGTTGA15115880.5024799173062275No Hit
GGCATCTTACCAGGCAGTCGCTCTCCCGGGGGCCTGTGCAGCCTGCAGCA14944120.4967702959943014No Hit
CATGGTGCCGCGCTTTACCGACGAGCAAGGGTCATCTGCAAACTCGGAGG14853470.4937569216817368No Hit
CTTGTGCAGTAGCCGCCTGCTGCTGCATCTGTCGATGACACTGACGCAAA14828110.49291390819506675No Hit
ACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGA14759410.4906301926377232No Hit
GCCTTTGACGAAGCTCATGCGGATGGTGCACATTCGGGTCAACTGGTAGA14664230.48746623271417217No Hit
CTAATACATTCTTGCTGCCTATGAATAATATAAATATATTTTCTAAGGAA14659090.48729536957058056No Hit
CAGATGGAGGTGATGCTGCAGTTGCTCATGCAGGATTTCTGGTTGTCACA14516600.4825587374051384No Hit
CTTGAATAAAAGACAATGTACTATGCTCAATTTTTTAAAGTAACTATCTG14300310.47536885621303016No Hit
CAGCCTGAATTATGATCCTTTCCTAGGTACCCCATGGCCTTAGGGAACCT14278300.4746372029464052No Hit
GTGCAAAAGAAATGCCAAGAAGGCCGAGTCGGGGAAGAGCGCCAGCACCG14193620.4718222825185181No Hit
GCAAGTGAGGTTAGAAAGAATTCCAGCTGCACAGGTGACCACATTTATAT14080270.4680543180581849No Hit
ATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGAGTGGGTCCCA13783520.4581897970735897No Hit
TCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTT13748610.4570293238551492No Hit
GGAAGCATGAGTACTGTGGTAAGTACTAAAACTATTAAGACAAGCCACTA13214610.4392781723613873No Hit
GGTTATCCATGAGCTTGAGATTGATTTTGTAGTCACCCACCTCTAAGGCC13113440.43591509371602427No Hit
AGATTTTGAGGGTGCCACCGGTGGTGAGCAGCAGGTTCCCCGGCTTGATG13034650.43329596782427615No Hit
GCAAATGAAAACCAAAACTTAAAAGAACAAAATCAAACAGAGAAATCTTT12895470.4286693662046099No Hit
AAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGAT12808530.4257793191805131No Hit
GGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTTCACAT12656920.4207395212816943No Hit
ATTTGGCCAGTGCTATCCTTGACCTGCCAGTCCTGGAGCACCTCTTTGCC12269490.40786062872883255No Hit
CCTATACACTGGAACATTTGCTTCATAAAGATCTTCAAGATTGGGCCACC11878280.39485608195753186No Hit
ATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTA11769810.3912503377580406No Hit
TGCCAAGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTTTGTGGGT11638840.3868966517821267No Hit
CATCTTGCTGAGGGGAAGGCTGGGCAGCTTGTTGCTGGCTGGCAGGCTGG11562520.38435963327650136No Hit
GGTCTTTCGAATGTATGCAATGTCATCAAAAGATTGTAGTTCTGGCATTC11318340.3762426367001966No Hit
GCTTCCGAGTCTCCCTTCAGCCCTCCACATCCCTACATTAATCCCTACAT11274880.3747979456067155No Hit
AGTACTTTGGCATAACTTCCTCTTCTAGTTTAGGACCTAGAGACTACTGC11104630.3691385194984515No Hit
CTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGCTC11080580.3683390528440976No Hit
TCAAAGATTCCAGAAGTCAGGACACGGCAGCGAAGCAGGTCCTGAAACAA10926580.3632198069076944No Hit
ACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCATAAAAAA10726050.35655381737764935No Hit
CACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATCACTGTGGAGGT10409170.3460201378170814No Hit
AAACCTCCAGACACACCTAGGCCTGTGATGACAGATCAATTTCCCAAATC10326650.3432770197997308No Hit
TCCTTCCCTGTCTAATTGCTTTTTTTTTTTTTGTAGTAATAAAAGAGCAG9936810.33031801437215No Hit
GTGATGTAGAATTTCAAGTTTTAAATCACAATGTGAAAAGTGCATAATTT9351110.31084825888545276No Hit
GTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCAT9303060.3092509876695815No Hit
GCAATCACTACTCCGCATTACTACATCCAAATCCATAAGAAGTTGTTTTT9254210.3076271229683263No Hit
CTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCT9250550.30750545777269483No Hit
CCACATGGGTTAATTTGCTTTTATAAAGGCTGCCTACTTTTTTCTCAACT9126720.30338911865383245No Hit
TGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATGCCAGT9110010.302833647227876No Hit
TCGTTTGGCATCATCATTATGCTTGCAAAAACGAACTAGATTTGACAGCC9093170.3022738543605446No Hit
GTTATAGCTGTTATTAAAGAATCCAATTCATCTTTTTTCTCCTTCAGCTT8968210.2981199519435774No Hit
TGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGC8392670.2789879337212558No Hit
GGATTGACTTCAACTGGGAATACCCTTCTTCGAAGGTAACGCTAATGATT8339790.2772301043373792No Hit
TCTTCACTCTGGGCGGCTCCCAAGGGTCATCCTGCTCTGGGGCAGCGCTT8163770.27137887271578376No Hit
TGTGCCTACCACGTAACCAAAAATGAAGGAGAACTACTGTTTACAAGCCG8058430.267877175527857No Hit
AATGACTGGACAAAAGGTGACTTATACTTACAATTTCATTGTAAGTGGGG8012390.2663467204440128No Hit
ACTGAGTTGCGATAATGTTTTCTTAATCCGCAATGCTGTAAGCCTAGCTG7987320.26551334709579444No Hit
ACAGTAACTGTGTATCTGTTTAAGTAATCAAAAAGGGATCCATGCTCATG7913380.2630554442091863No Hit
TCTTGGTGGATGACGGGCCGGAGCCGAGCAGCTGAAGGCACCCGCTGGGT7810520.25963618682594963No Hit
ACCAATTCCAGGTGATACAACTCGTTCGTAGTGATATGGATTCACACAGA7539620.2506309678378222No Hit
GGATGATTGGAAATGGGAGGCTGGAATGCAAGCTCATTGTGAACAGGCCC7193590.2391282881535779No Hit
GCCCAACGCACCGAATAGTTACGGTCGGAGGCCGATCCAGGTGGGTAGAG7055380.23453393113493964No Hit
GCATGTATAGCACCTCGAGTGTTGAGATTACAAGCAGCACATCAGGTAAA7003900.23282264035048486No Hit
AGGAGAGGGATAGGAAGAGCTCACATGGTGAGGCTCACTCCCCCTCCTCT6912580.2297869939939112No Hit
CATGTGTGACTTGATTAGCAGGTCAAAAGTGAACTGATGCAGCTCTCTCG6887210.22894364808867385No Hit
CACTATTATCTACCATGAAGTGCGAATAGTGGTGAGAGACAGGAATGACA6663290.22150013152971662No Hit
AGGTACTTTGTTGTTCAGTTTACTCTCAAAATTGCTCATAATGAAAGACA6617200.21996801435303592No Hit
TTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAG6593600.2191835065342105No Hit
GTCTGTAAGGGCCTCGTGGCTGGGCCTCTGTGCTCCCCATCCCCCCACCC6492030.21580713114615546No Hit
GCACTTTAGGATAACAAAGTCATACGAATTACATACCTTGAGAAAAGCTC6346070.21095514973786053No Hit
GCAAATGCTATCGATTTCTTGATCACATAGACTTCCATTTTCTACTTTTT6228270.20703925901507936No Hit
AAGCAGTCTGTGTCCTGAGGGCCTGAGCAGCCCCCGGCACACTCATCATG6111810.20316791238031623No Hit
AGGATGAGGACAGTGAAGGCCCAGGGAGTGGAGAGAGTCGCCTACAGCAT6056740.2013372833302011No Hit
TCATGTCCTCGGAGCAGCAGCACCACCACAGCCACCACCACCACCAGCCC6055870.20130836291484613No Hit
ATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTA6041710.20083765822355085No Hit
TCCTGAAGGCTTGCTCTCTTTGGTTAAGGTAATGCCTTGTTGTCCACCTG5981630.198840487471219No Hit
ATCAGTGCTTTGGCCATTGCTGCCTTGGCTGAAGCAGCAACTCCTTATGG5851850.1945263593048137No Hit
TCAAAGGCATAGAGACACTGGATATACTGCTTTTTCAAGGAGCTGGCAGC5816180.1933406222752585No Hit
GGGGTAGTTAGGGCAGGAAATGCATTATCTCAGAGGATGGCTCCACAAGA5778150.19207643446382075No Hit
CTGTCGCAACCGCAATGTGCGGGAAGCCCTGCAGGCCTGGGCGGACACGG5743240.19091596124538024No Hit
GGAAGATAAACTAAAACGAGAAGAAGAAAAAAGAGGTTGGTTTCTTTTCA5681480.18886294417374216No Hit
AGCTAAAGGATTAATGGCACCTAAAAATAAAAAGCACTCATTAACACTAA5668040.18841617361929242No Hit
AGGCACAAGGGGTCTGAGTACCTGTGAGATGGCCCACTGGGCCGCAGCAA5478680.18212149562892022No Hit
TCTGCTCACCTCACTGTACCGCAGGGTGAGTGGATGTGGTATTATACCTG5425950.18036865252355305No Hit
TCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTGTCTGGCTCAGCCTGGA5312520.1765980287146815No Hit
CCAGCTTGCCAGATTTTTCCCCATTACACCCGCAGTGTGGCATATCCTTG5222760.17361423965460646No Hit
CCGTGTCATAGAGCAGCGCATCCCCTACCGTGGCACCCGCTACCCAGAGG5141680.1709189899109469No Hit
CCAGCTTGCCAGATTTTTCCCCATTACACCCCCAGTGTGGCATATCCTTG5113490.16998190196973126No Hit
TCCTTCCCTGTCTAATTGCTTTTTTTTTTTTGTAGTAATAAAAGAGCAGT4940410.1642284014069217No Hit
AAAACTTGATAGGCAGCTTCAATTTTTTTTCTTTATTTTCAGTAATCTAT4914260.16335912685343504No Hit
TTAAAAATAGGGTTTGGCTGCTTTCTTGAAGGCTATTGGGTATCTTATTC4773540.15868133277440474No Hit
CGCACAAGAATCAGGTAGGAAAAAAAAAAAAAGACTCACCAAACTCATCG4735170.15740584273167882No Hit
TGAGGGTTTCCAAGACCAGTCTCTGCGGGGAAAGGACGGCATTGGGGCCC4717800.15682843168028063No Hit
CTGCTGGACCCTGGTGTTCACCTGTTGAGCTGTCTTGGTCTTGTGCTTCT4536940.1508163094721125No Hit
CTCCAGTACAAGATGAGGAAAATTACTACAAAGAAAAACAAAAAGCTTCA4449620.1479136305424584No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTAAATAGAG4442660.14768226721961836No Hit
TTCTTGAGGATCTTGACGGCCCTCCTGCACAGCGTCTCCGAGTCCAGCAC4416630.1468169816889393No Hit
ATCCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGCTGCATCC4248800.14123800087396166No Hit
GGTAGCATTAGACTCAGATGGGGCTAACAGAGCTGGGGTGCTGTATGTCT4212730.14003896710171448No Hit
GCCAAAGTCCTCGGACACTTCCACTCTCTTTTCAGGAGGCACAAAGATGG4175410.13879838100855493No Hit
AGTATATATCCGAGCCGCTGTCCGAAAAGAGAAAGGCCTGCCCATCCTCG3854840.12814203899665372No Hit
TTCTCTGCGTTCAGCTGCACGGTCCCGGTACTTCATTTGCTGAAAGTTTG3738530.12427567864040008No Hit
CGACAAGAGATTGAGAAGGAGAAAAAAGTGGAGCCATGGAAAGAACAATT3737960.12425673078206405No Hit
ACATTGGAGAGCAGCCCTAGGCAGAAGCGCTCCGAATTGGAGGGGTCGGT3636320.1208780284640379No Hit
CCACAGATTTTGCATCCTTAGCAACCCCTGCTTGTTCTGACTCCTGTTTT3378490.11230728054337005No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTCCCCCAGAACGGAG3358070.11162848182894568No Hit
AGGTCCAGTTCCCATCATGCCCCCTTGATTCATATTGGGGTAGCCTGGCG3266440.10858253049678575No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3068180.10199199998151753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)