FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007680878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007680878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145297681
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTGGAGTATCTGTTTGCTCAGGTAGGAAGTTTGGGCCCAGGAGAA23216061.597827290856762No Hit
GACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGAGACCGG22000271.5141514887632652No Hit
AGTACTTTGGCATAACTTCCTCTTCTAGTTTAGGACCTAGAGACTACTGC19063811.3120519108629132No Hit
CCAATAATCTCTTAAAACAGGCCTCTTTTTTTTATGCACAACAACTTCCT18576331.2785014786299307No Hit
TGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTA18406131.266787595873605No Hit
CCCTTTCCTCAGAATTACTCAGCTGCGGAGGATCTCCTTTCACGACTTGT18208911.2532140826115457No Hit
TGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCAC17530221.2065037706967945No Hit
TTGGGTAATCCGGTCCCCAGCCTTTCACAAAACTCATCCTGAGTATGCAT17135861.1793622501105163No Hit
CATATTCATGGAGAGAAGAAGGAGTTCGTGTGCAGGTGGCTGGACTGCTC16780001.1548704621101282No Hit
CTTTTGTACAGAGGTGGCAGAAACACTGTAACGCTGGAAAAAGAGAAAGA16149081.1114478833285715No Hit
GTCTGGCTCCGGTAAGTAAGAGTGCCAACCAAAAATGCAAGTGCACCATC15354231.0567429496689627No Hit
CACACCTACCTCCCACTGCATTACAGCGAGATGTCATTTCCCAGAGCACC14757381.0156652121653615No Hit
CCCAGCCTCCAATTACGCGGACCCCTACCGACAGCTGCAGTATTGTCCCT14206850.9777754126715897No Hit
AGAACCATAAGCTCCTCGTCCAATTTCTCCAAGGTCTTTCAAGTCCTCTG14158960.9744794206316342No Hit
GTATCGTCGTCCTCCCCGCAGTCAGGCTGCCCATCACCCACCATTCCAGC13941140.9594881283755657No Hit
GTTGCCAAGCCAAAGTCTGAGATTTTGAGGTTATCTAAACCAAAAGAAAA13457580.9262074870967831No Hit
ACTTCTTCTTGCGTTCCTCAGTACTGGGTGGAGCAGCCCCCGGTTTGTCA13398260.9221248341878217No Hit
TGTCAGAAGAACCCCTGGCCCCCTCCAGCATAGATGCTGAAAGCAATGGA11590750.7977243628547657No Hit
ACAAATGTTTCAGAACTTCAAACTATGTGCCCTTCAGAAGTAACAAATAT11494470.7910979666633495No Hit
CTGTGCCCCTAACTGTGTGGCCGAAGTCGTGACATTTGACAAAGAGGACA11255570.7746558597862274No Hit
CATGTGTGACTTGATTAGCAGGTCAAAAGTGAACTGATGCAGCTCTCTCG11023240.7586659280542819No Hit
TTTGGTGAATGGCTGGTGCATAAGTTTGACCTTGTTGAGCTGGCTGGCTT10984870.7560251426173829No Hit
CTCTGTTTTCCTGCTCCTATCAGGTGTGTGTCCTGTATGCAGTGTGGGGC10882090.7489513889764008No Hit
GCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATGTGTG10735120.7388362929206007No Hit
ATGGAAACCTTGCGCCCTCGCTCTAGAGAGCTGGTGTCCATTTCACTGTC10664930.7340055207075191No Hit
CTTCCGTGGCAGATTGTGCAGGTATAGACCTTGATCCAAGGGGAGGGCAG10497560.7224864105023121No Hit
CCTTTCAGTTTCTCTTGTAGTCTCATTAAGACACGTTCAGCTACTTTGTT10251690.7055645987908093No Hit
GCCTGTAGTAATCAAGTGTCTCATTTTTTCATTTCTTGTAGCAGAAACTT9944910.684450703655759No Hit
TATGCTCAGTGGTGTCCCCCCCACCAATCCGCCAGTGCAGCAGGCCACTG9866480.6790528198450738No Hit
TACTTTCCCGTGGCCGACCTGTCTATCATCGCCGCCCTCTATGCCCGCTT9556450.6577152459852404No Hit
GTACATGAACTTGTCTTCCCGTCGTGTGGGTCCTGAATTGGAGGAATATA9519020.655139155317971No Hit
CTCAACAGTCATCAACCACTACCGCATGCGGGGCCATAGCCCCTTTGCCA9421520.6484287935744825No Hit
TGGGTGAAATGGCAACTGTTGCCCCAGTTCTCTACACCAATATTAATTTC8968360.617240408675208No Hit
AAACCTCCAGACACACCTAGGCCTGTGATGACAGATCAATTTCCCAAATC8919320.6138652687787908No Hit
GGTTAGAATTTCTAAGAAGTTATCCTGTTTTTTTTCTCTCTTAGATCTGT8885990.6115713574258629No Hit
GTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGAGACAACATCAT8883950.6114309560109221No Hit
TCCAAGGAAGGCAGTGACCTGTCAGTGGTGGAGCGTGCAGAAGTCTGGCT8760250.602917399624568No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTTAAATAGA8550100.5884539891589874No Hit
GCCCAGCACTTGAGGGTACCTGGCACTGCAGCAGGCGAGAGTGGTCCTGA8405020.5784689708846764No Hit
ATTTGGCCAGTGCTATCCTTGACCTGCCAGTCCTGGAGCACCTCTTTGCC8267800.5690249110032252No Hit
CACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATCACTGTGGAGGT8264480.5687964145828315No Hit
TGCCAAGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTTTGTGGGT8249530.5677674924488298No Hit
GGGCTATCTCACTTCTCAAGAACCAAAACAAAAAGTGACCAATTCTTTTG8239920.5671060916657025No Hit
ACGTCTTTGGTGTGCTGCAGGCCAAGCAAGGCCAGGAAGCCCTTCTGGTC8113570.5584101510883714No Hit
ATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTA7983040.549426525258858No Hit
CCTATACACTGGAACATTTGCTTCATAAAGATCTTCAAGATTGGGCCACC7935640.5461642570881775No Hit
GCCAAGAATCAGCAAGCCATGGATAGCTGGGATCACCAGCATTGAGAAGA7933610.546024543915467No Hit
CATCTTTGTGATCATCCTGGCTTCGGTGCTGCGCATCCGGTGCCGCCCCC7916170.54482424946617No Hit
CCATGCCTACTAGCCTCCCTAACCTTGCCAAGGAGGCAAAGGATGCCAAG7818750.5381193936605223No Hit
GCAGGGGCATACTAATAGAGTCTATTCATTACAGGTAAGATCTCTTATCT7803350.5370595006261661No Hit
CCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGA7758390.5339651635596304No Hit
ATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGAGTGGGTCCCA7739900.5326926036761729No Hit
GGAAGTCATTGAATCCTTTGCCATTAACATGGAAACAGGCTGGATTACAA7704950.5302871970819686No Hit
CCAATTGCCTTCTGGTCATATCTGTTTGTAAGGAAAACTAAAACCACAAA7663100.5274069033489943No Hit
GGACTTCTGGTCGAACATGCCGGCCTGGATCACCTTCTGGTCCACGTTGA7550360.5196476604468312No Hit
ATTCCTCACAGCATTGCCCATGACGCCGAGCCCTGCTCTGACCCTAGGAC7512220.5170227045812245No Hit
GGGAGACCTCTTAGATCAGTTCTTTTGTACTACTTGTGGTCAGCACTATC7497300.5159958471739132No Hit
CAGATGCAATGATCCTTGTGGATGGACAGCCACGCCAGGCCAAGGGTGAG7285670.5014305768582775No Hit
ACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGA7177060.4939555779971464No Hit
ATCCTTATCTCCCTTGCTTGGAGAGCCAGCCCTGTCTGAGCCTGGGGAAC7168280.4933513013191174No Hit
CTTGAATAAAAGACAATGTACTATGCTCAATTTTTTAAAGTAACTATCTG7156310.49252747536968605No Hit
CACATGCCATTTTTCTTCACCAGAATGTTCTTTGACTTTAAGTCTCGATG7132310.49087569401744274No Hit
AGGATGTTGTAATAGGGTGGCTTAGGTGTAACTGGATTTGAAGTGGCTGT7095000.4883078622569344No Hit
CCAGCTTGCCAGATTTTTCCCCATTACACCCCCAGTGTGGCATATCCTTG7005870.4821735592600408No Hit
GGTTATCCATGAGCTTGAGATTGATTTTGTAGTCACCCACCTCTAAGGCC6964570.479331118849722No Hit
TCCTGCAAGCAGCACAAAACCGAGGACCAGCTTTACCTGGCCCGTTGGTA6859970.4721321051228616No Hit
CATGGTGCCGCGCTTTACCGACGAGCAAGGGTCATCTGCAAACTCGGAGG6676970.45953727231200614No Hit
CTTTGAAAACGGTCCTGCAAACCGCCACAAGCCATTAAAACCAAAACCTG6567510.4520037728613164No Hit
TCAACAAGATGTTTTGCCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGG6460590.44464508693707233No Hit
AAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGAT6383000.4393050154737156No Hit
GGATTCATCAGCATCGTCAAGCTGGAGAGTCCTCGACGGGCCCCCCGCCC6255010.430496203170648No Hit
CTAATACATTCTTGCTGCCTATGAATAATATAAATATATTTTCTAAGGAA6221480.428188526973118No Hit
GTGCAAAAGAAATGCCAAGAAGGCTGAGTCGGGGAAGAGCGCCAGCACCG6210630.42744178415345807No Hit
GTGATGTAGAATTTCAAGTTTTAAATCACAATGTGAAAAGTGCATAATTT6185220.42569296064677037No Hit
CGCTCAGGCATTCCTCCGATCGCACACATTTGTCGTGGCACCAGCCACAC6181600.4254438169594737No Hit
GGCATCTTACCAGGCAGTCGCTCTCCCGGGGGCCTGTGCAGCCTGCAGCA5990800.4123121552091392No Hit
GCTTCTCAATACCCTGTGTCTCTCGAAGCTTTTTGGCAGCCTCCAAGTTC5893300.40560179346565073No Hit
ACATTTTTGTCTTACCTAGTTCATTCATTGCATGTCTATGCTCTTCATCA5805940.39958930934348497No Hit
GCGATACACTCTACAGCAGACTGCCAGTCCTTGTAATTTTTATCAAAGTT5790430.3985218456445977No Hit
GCCTTTGACGAAGCTCATGCGGATGGTGCACATTCGGGTCAACTGGTAGA5727180.39416871353920646No Hit
CAGCCTGAATTATGATCCTTTCCTAGGTACCCCATGGCCTTAGGGAACCT5624420.3870963363826846No Hit
GCAAGTGAGGTTAGAAAGAATTCCAGCTGCACAGGTGACCACATTTATAT5622600.3869710762968061No Hit
CAGATGGAGGTGATGCTGCAGTTGCTCATGCAGGATTTCTGGTTGTCACA5541920.38141833798434815No Hit
CATCTTGCTGAGGGGAAGGCTGGGCAGCTTGTTGCTGGCTGGCAGGCTGG5377980.370135294864066No Hit
CTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGCTC5336210.3672605070689325No Hit
AGATTTTGAGGGTGCCACCGGTGGTGAGCAGCAGGTTCCCCGGCTTGATG5327320.3666486597263724No Hit
CCTCCAGCGACTATACTGAATACAGTGAAGAAGAGTCTGAGTTCAGTGAG5318090.3660134121479888No Hit
AGGTACTTTGTTGTTCAGTTTACTCTCAAAATTGCTCATAATGAAAGACA5310000.3654566241838368No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTCCCCCAGAACGGAG5290330.36410285171722734No Hit
CTCTACTGTGTGCCTCTGCAGGCTCGGATGGCGGGTGAGCGAGGAGCCAG5141960.353891401749213No Hit
TGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATGCCAGT5120760.35243232822139814No Hit
CTTGTGCAGTAGCCGCCTGCTGCTGCATCTGTCGATGACACTGACGCAAA5047720.3474054069727376No Hit
GCAAATGAAAACCAAAACTTAAAAGAACAAAATCAAACAGAGAAATCTTT5037630.3467109705625653No Hit
AGGATGAGGACAGTGAAGGCCCAGGGAGTGGAGAGAGTCGCCTACAGCAT4998980.3440509143432234No Hit
ACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCATAAAAAA4987470.3432587475363767No Hit
GGTCTTTCGAATGTATGCAATGTCATCAAAAGATTGTAGTTCTGGCATTC4980120.3427528894972522No Hit
TGAGGGTTTCCAAGACCAGTCTCTGCGGGGAAAGGACGGCATTGGGGCCC4938100.3398608956463662No Hit
CCAGGATAGTCTTTCTCAGGCTCAGACTTCTCAGCCACCCTCACCGCAAG4931260.3393901379609768No Hit
AGGCACAAGGGGTCTGAGTACCTGTGAGATGGCCCACTGGGCCGCAGCAA4906290.3376715971124137No Hit
GGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTTCACAT4885180.33621871776466966No Hit
TCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTT4836550.33287179579968657No Hit
GGAAGCATGAGTACTGTGGTAAGTACTAAAACTATTAAGACAAGCCACTA4828740.3323342786179774No Hit
GGATGATTGGAAATGGGAGGCTGGAATGCAAGCTCATTGTGAACAGGCCC4815980.33145608153236805No Hit
GCTTCCGAGTCTCCCTTCAGCCCTCCACATCCCTACATTAATCCCTACAT4809500.3310101005672623No Hit
TGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGC4603930.3168619050430681No Hit
TTAAAAATAGGGTTTGGCTGCTTTCTTGAAGGCTATTGGGTATCTTATTC4594490.3162122043778524No Hit
ATCAGTGCTTTGGCCATTGCTGCCTTGGCTGAAGCAGCAACTCCTTATGG4420920.3042663839899826No Hit
TCCTTCCCTGTCTAATTGCTTTTTTTTTTTTTGTAGTAATAAAAGAGCAG4373710.30101719242167396No Hit
ATTGAACTCCAGCTGCCTGAGGTGATGGGGACTTGGAAGGGACACAGGGG4369890.3007542838897752No Hit
TCAAAGATTCCAGAAGTCAGGACACGGCAGCGAAGCAGGTCCTGAAACAA4359520.3000405766971601No Hit
TCTTCACTCTGGGCGGCTCCCAAGGGTCATCCTGCTCTGGGGCAGCGCTT4283520.29480993574838954No Hit
GTCTGTAAGGGCCTCGTGGCTGGGCCTCTGTGCTCCCCATCCCCCCACCC4198480.28895712382360733No Hit
GGGGTAGTTAGGGCAGGAAATGCATTATCTCAGAGGATGGCTCCACAAGA4191780.2884960015294394No Hit
GCCCAACGCACCGAATAGTTACGGTCGGAGGCCGATCCAGGTGGGTAGAG4183850.28795022544096904No Hit
GCAATCACTACTCCGCATTACTACATCCAAATCCATAAGAAGTTGTTTTT4161920.28644091023035667No Hit
TCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTGTCTGGCTCAGCCTGGA4093560.2817360863453836No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTTCCCCCAGAACGGA4058530.27932517381333843No Hit
TGTGCCTACCACGTAACCAAAAATGAAGGAGAACTACTGTTTACAAGCCG3922830.2699857267508626No Hit
GGTAGCATTAGACTCAGATGGGGCTAACAGAGCTGGGGTGCTGTATGTCT3833200.26381701164246385No Hit
GTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCAT3798630.26143775825300336No Hit
CCACATGGGTTAATTTGCTTTTATAAAGGCTGCCTACTTTTTTCTCAACT3771760.2595884513807209No Hit
TCCTGAAGGCTTGCTCTCTTTGGTTAAGGTAATGCCTTGTTGTCCACCTG3771000.25953614497123323No Hit
TCAAAGGCATAGAGACACTGGATATACTGCTTTTTCAAGGAGCTGGCAGC3748950.25801857085385965No Hit
CCTCCAGCGACTATACTGAATACAGTGAAGAAGCGTCTGAGTTCAGTGAG3700930.2547136316649128No Hit
AATGACTGGACAAAAGGTGACTTATACTTACAATTTCATTGTAAGTGGGG3586710.24685252891269477No Hit
CTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCT3555340.2446935130368667No Hit
CCGTGTCATAGAGCAGCGCATCCCCTACCGTGGCACCCGCTACCCAGAGG3443740.23701272974893522No Hit
GTTATAGCTGTTATTAAAGAATCCAATTCATCTTTTTTCTCCTTCAGCTT3398720.2339142632290188No Hit
CTGCTGGACCCTGGTGTTCACCTGTTGAGCTGTCTTGGTCTTGTGCTTCT3284240.22603526617881808No Hit
AGCTAAAGGATTAATGGCACCTAAAAATAAAAAGCACTCATTAACACTAA3127840.2152711577000324No Hit
GCATGTATAGCACCTCGAGTGTTGAGATTACAAGCAGCACATCAGGTAAA3042560.20940182796172777No Hit
ACTGAGTTGCGATAATGTTTTCTTAATCCGCAATGCTGTAAGCCTAGCTG2991620.2058959220415913No Hit
ATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTA2908320.20016286426484675No Hit
TTCTCTGCGTTCAGCTGCACGGTCCCGGTACTTCATTTGCTGAAAGTTTG2898410.19948081621481628No Hit
GCACTTTAGGATAACAAAGTCATACGAATTACATACCTTGAGAAAAGCTC2832850.19496870015427156No Hit
GGAAGATAAACTAAAACGAGAAGAAGAAAAAAGAGGTTGGTTTCTTTTCA2736530.188339550993935No Hit
GGATTGACTTCAACTGGGAATACCCTTCTTCGAAGGTAACGCTAATGATT2733890.18815785504518823No Hit
ACAGTAACTGTGTATCTGTTTAAGTAATCAAAAAGGGATCCATGCTCATG2715780.1869114483664746No Hit
ACCAATTCCAGGTGATACAACTCGTTCGTAGTGATATGGATTCACACAGA2670370.1837861403995842No Hit
TCGTTTGGCATCATCATTATGCTTGCAAAAACGAACTAGATTTGACAGCC2651650.18249775094483442No Hit
TCTTGGTGGATGACGGGCCGGAGCCGAGCAGCTGAAGGCACCCGCTGGGT2608360.17951835033072552No Hit
CCACAGATTTTGCATCCTTAGCAACCCCTGCTTGTTCTGACTCCTGTTTT2519570.1734074475696553No Hit
GCAAATGCTATCGATTTCTTGATCACATAGACTTCCATTTTCTACTTTTT2517070.17323538701212995No Hit
AGGTCCAGTTCCCATCATGCCCCCTTGATTCATATTGGGGTAGCCTGGCG2364380.16272661640071187No Hit
TTCTTGAGGATCTTGACGGCCCTCCTGCACAGCGTCTCCGAGTCCAGCAC2345440.16142308561689983No Hit
AGGAGAGGGATAGGAAGAGCTCACATGGTGAGGCTCACTCCCCCTCCTCT2341580.1611574241160807No Hit
GCCAAAGTCCTCGGACACTTCCACTCTCTTTTCAGGAGGCACAAAGATGG2334310.16065707201479698No Hit
AAGCAGTCTGTGTCCTGAGGGCCTGAGCAGCCCCCGGCACACTCATCATG2305030.15864189876506013No Hit
TCCTTCCCTGTCTAATTGCTTTTTTTTTTTTGTAGTAATAAAAGAGCAGT2228130.15334931601558047No Hit
CACTATTATCTACCATGAAGTGCGAATAGTGGTGAGAGACAGGAATGACA2138260.1471640830936593No Hit
GTACAGATGTATTTGGACTCATTCAATATTGCCCCGTTATAGAACCACTG2126470.14635264550436974No Hit
CTGTCGCAACCGCAATGTGCGGGAAGCCCTGCAGGCCTGGGCGGACACGG2098890.14445447343375012No Hit
TTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAG2095340.14421014744206415No Hit
GTCACAGAGCTTAATATTTCCACTTCTGTCCAGAAGAATATTGGAAGGTT2008900.13826098160506775No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTAAATAGAG1978140.13614394850527586No Hit
TGCTGCCGGATGTGGTACTGCTGCTGCTGCTGCTGCTGTTGCTGTTGCTG1941770.13364081151439713No Hit
CTCCAGTACAAGATGAGGAAAATTACTACAAAGAAAAACAAAAAGCTTCA1822330.12542044631806615No Hit
CGCACAAGAATCAGGTAGGAAAAAAAAAAAAAGACTCACCAAACTCATCG1788980.12312515848067804No Hit
GCTCTTGTCTTTCAGACTTCCTGAAAACAACGTTCTGGTAAGGACAAGGG1784030.12278447857677784No Hit
CGACAAGAGATTGAGAAGGAGAAAAAAGTGGAGCCATGGAAAGAACAATT1779530.12247476957323222No Hit
ACATTGGAGAGCAGCCCTAGGCAGAAGCGCTCCGAATTGGAGGGGTCGGT1670640.1149804999296582No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1641170.11295225007754942No Hit
TCATGTCCTCGGAGCAGCAGCACCACCACAGCCACCACCACCACCAACCC1592780.1096218459260888No Hit
TCATGTCCTCGGAGCAGCAGCACCACCACAGCCACCACCACCACCAGCCC1583900.10901068682575878No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)