FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007680886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007680886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119291330
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTGGAGTATCTGTTTGCTCAGGTAGGAAGTTTGGGCCCAGGAGAA20330061.7042361754202924No Hit
TGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCAC18907091.5849508929106582No Hit
GACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGAGACCGG16394401.3743161384821512No Hit
CCAATAATCTCTTAAAACAGGCCTCTTTTTTTTATGCACAACAACTTCCT15566741.3049347341504198No Hit
GTCTGGCTCCGGTAAGTAAGAGTGCCAACCAAAAATGCAAGTGCACCATC15197591.2739894843992434No Hit
CCCTTTCCTCAGAATTACTCAGCTGCGGAGGATCTCCTTTCACGACTTGT14834201.2435270861679555No Hit
GTTGCCAAGCCAAAGTCTGAGATTTTGAGGTTATCTAAACCAAAAGAAAA14403371.2074113013913081No Hit
CTTTTGTACAGAGGTGGCAGAAACACTGTAACGCTGGAAAAAGAGAAAGA14149481.1861281117412306No Hit
ACAAATGTTTCAGAACTTCAAACTATGTGCCCTTCAGAAGTAACAAATAT13837401.1599669481428365No Hit
TGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTA13421941.1251396057031136No Hit
AGGATGTTGTAATAGGGTGGCTTAGGTGTAACTGGATTTGAAGTGGCTGT12796461.072706625033018No Hit
TGGGTGAAATGGCAACTGTTGCCCCAGTTCTCTACACCAATATTAATTTC12619401.057863970499784No Hit
TTGGGTAATCCGGTCCCCAGCCTTTCACAAAACTCATCCTGAGTATGCAT12320921.0328428729900152No Hit
GCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATGTGTG12307471.0317153811597206No Hit
CCCAGCCTCCAATTACGCGGACCCCTACCGACAGCTGCAGTATTGTCCCT12219101.0243074664353227No Hit
CATATTCATGGAGAGAAGAAGGAGTTCGTGTGCAGGTGGCTGGACTGCTC12217531.0241758558647975No Hit
CTGTGCCCCTAACTGTGTGGCCGAAGTCGTGACATTTGACAAAGAGGACA12022721.00784524742913No Hit
CCTTTCAGTTTCTCTTGTAGTCTCATTAAGACACGTTCAGCTACTTTGTT12017411.007400118684233No Hit
CTTCCGTGGCAGATTGTGCAGGTATAGACCTTGATCCAAGGGGAGGGCAG11627660.9747280041223448No Hit
GTATCGTCGTCCTCCCCGCAGTCAGGCTGCCCATCACCCACCATTCCAGC11620770.974150426523034No Hit
CTCTGTTTTCCTGCTCCTATCAGGTGTGTGTCCTGTATGCAGTGTGGGGC11577180.9704963470522123No Hit
TACTTTCCCGTGGCCGACCTGTCTATCATCGCCGCCCTCTATGCCCGCTT11438590.9588785706387883No Hit
AGAACCATAAGCTCCTCGTCCAATTTCTCCAAGGTCTTTCAAGTCCTCTG10583120.8871658988125961No Hit
ATCCTTATCTCCCTTGCTTGGAGAGCCAGCCCTGTCTGAGCCTGGGGAAC10566930.8858087171967988No Hit
GGTTAGAATTTCTAAGAAGTTATCCTGTTTTTTTTCTCTCTTAGATCTGT10303070.8636897585096922No Hit
CCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGA9416370.7893591260991053No Hit
GGGCTATCTCACTTCTCAAGAACCAAAACAAAAAGTGACCAATTCTTTTG9350760.7838591455053775No Hit
TTTGGTGAATGGCTGGTGCATAAGTTTGACCTTGTTGAGCTGGCTGGCTT9329750.7820979110552292No Hit
TGTCAGAAGAACCCCTGGCCCCCTCCAGCATAGATGCTGAAAGCAATGGA8888790.745132944699334No Hit
GGGAGACCTCTTAGATCAGTTCTTTTGTACTACTTGTGGTCAGCACTATC8854070.7422224230377849No Hit
CCTCCAGCGACTATACTGAATACAGTGAAGAAGAGTCTGAGTTCAGTGAG8744950.733075069244345No Hit
ATGGAAACCTTGCGCCCTCGCTCTAGAGAGCTGGTGTCCATTTCACTGTC8649970.7251130488695197No Hit
CTTTGAAAACGGTCCTGCAAACCGCCACAAGCCATTAAAACCAAAACCTG8423850.7061577735783481No Hit
CCAATTGCCTTCTGGTCATATCTGTTTGTAAGGAAAACTAAAACCACAAA8337640.6989309281739083No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTTCCCCCAGAACGGA8252470.6917912642938929No Hit
TCCTGCAAGCAGCACAAAACCGAGGACCAGCTTTACCTGGCCCGTTGGTA8233520.6902027163248159No Hit
CATCTTTGTGATCATCCTGGCTTCGGTGCTGCGCATCCGGTGCCGCCCCC8169870.6848670393732721No Hit
AGATTTTGAGGGTGCCACCGGTGGTGAGCAGCAGGTTCCCCGGCTTGATG8024290.6726633025216502No Hit
GCCTGTAGTAATCAAGTGTCTCATTTTTTCATTTCTTGTAGCAGAAACTT7875900.6602240078973048No Hit
TATGCTCAGTGGTGTCCCCCCCACCAATCCGCCAGTGCAGCAGGCCACTG7850260.6580746480066908No Hit
ACTTCTTCTTGCGTTCCTCAGTACTGGGTGGAGCAGCCCCCGGTTTGTCA7779020.6521027135836276No Hit
GTACATGAACTTGTCTTCCCGTCGTGTGGGTCCTGAATTGGAGGAATATA7759220.6504429114840115No Hit
CTCAACAGTCATCAACCACTACCGCATGCGGGGCCATAGCCCCTTTGCCA7538290.6319227055310725No Hit
CAGCCTGAATTATGATCCTTTCCTAGGTACCCCATGGCCTTAGGGAACCT7450910.6245977809116555No Hit
GTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGAGACAACATCAT7402540.6205430017420377No Hit
TCTTCACTCTGGGCGGCTCCCAAGGGTCATCCTGCTCTGGGGCAGCGCTT7330890.614536697679538No Hit
GGAAGTCATTGAATCCTTTGCCATTAACATGGAAACAGGCTGGATTACAA7249430.6077080371222284No Hit
GCCAAGAATCAGCAAGCCATGGATAGCTGGGATCACCAGCATTGAGAAGA7050420.591025349453309No Hit
CGCTCAGGCATTCCTCCGATCGCACACATTTGTCGTGGCACCAGCCACAC7025470.5889338311510149No Hit
TCCAAGGAAGGCAGTGACCTGTCAGTGGTGGAGCGTGCAGAAGTCTGGCT7022650.588697435094403No Hit
GCAGGGGCATACTAATAGAGTCTATTCATTACAGGTAAGATCTCTTATCT6802590.5702501598397804No Hit
GCGATACACTCTACAGCAGACTGCCAGTCCTTGTAATTTTTATCAAAGTT6791410.5693129584522194No Hit
GGCATCTTACCAGGCAGTCGCTCTCCCGGGGGCCTGTGCAGCCTGCAGCA6774090.5678610507569997No Hit
CACATGCCATTTTTCTTCACCAGAATGTTCTTTGACTTTAAGTCTCGATG6657340.5580740863564854No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTTAAATAGA6604670.5536588451147288No Hit
GCTTCTCAATACCCTGTGTCTCTCGAAGCTTTTTGGCAGCCTCCAAGTTC6602340.5534635249686628No Hit
GGACTTCTGGTCGAACATGCCGGCCTGGATCACCTTCTGGTCCACGTTGA6567210.5505186336676773No Hit
CAGATGCAATGATCCTTGTGGATGGACAGCCACGCCAGGCCAAGGGTGAG6486780.5437763163509033No Hit
CCAGGATAGTCTTTCTCAGGCTCAGACTTCTCAGCCACCCTCACCGCAAG6477870.543029405406076No Hit
ACATTTTTGTCTTACCTAGTTCATTCATTGCATGTCTATGCTCTTCATCA6475370.5428198344339022No Hit
GCCCAGCACTTGAGGGTACCTGGCACTGCAGCAGGCGAGAGTGGTCCTGA6395680.5361395501248917No Hit
CTAATACATTCTTGCTGCCTATGAATAATATAAATATATTTTCTAAGGAA6371830.5341402430503541No Hit
GCCTTTGACGAAGCTCATGCGGATGGTGCACATTCGGGTCAACTGGTAGA6293350.5275613910918756No Hit
TCAACAAGATGTTTTGCCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGG6262970.5250146846380201No Hit
GTGCAAAAGAAATGCCAAGAAGGCCGAGTCGGGGAAGAGCGCCAGCACCG6190060.5189027568055449No Hit
ACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGA6044690.5067166239155855No Hit
CAGATGGAGGTGATGCTGCAGTTGCTCATGCAGGATTTCTGGTTGTCACA5958590.4994989996339214No Hit
GGATTCATCAGCATCGTCAAGCTGGAGAGTCCTCGACGGGCCCCCCGCCC5920360.4962942403274404No Hit
ATTCCTCACAGCATTGCCCATGACGCCGAGCCCTGCTCTGACCCTAGGAC5894190.49410045139072556No Hit
ACGTCTTTGGTGTGCTGCAGGCCAAGCAAGGCCAGGAAGCCCTTCTGGTC5892300.4939420157357622No Hit
GGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTTCACAT5750520.4820568267618443No Hit
GCAAGTGAGGTTAGAAAGAATTCCAGCTGCACAGGTGACCACATTTATAT5646180.47331017266720055No Hit
GCAAATGAAAACCAAAACTTAAAAGAACAAAATCAAACAGAGAAATCTTT5555720.46572705661006547No Hit
CTCTACTGTGTGCCTCTGCAGGCTCGGATGGCGGGTGAGCGAGGAGCCAG5432800.45542287105022633No Hit
CACACCTACCTCCCACTGCATTACAGCGAGATGTCATTTCCCAGAGCACC5321210.44606846113627874No Hit
ATTTGGCCAGTGCTATCCTTGACCTGCCAGTCCTGGAGCACCTCTTTGCC5219140.4375120974843687No Hit
CTTGTGCAGTAGCCGCCTGCTGCTGCATCTGTCGATGACACTGACGCAAA5201140.4360031864847177No Hit
CATGGTGCCGCGCTTTACCGACGAGCAAGGGTCATCTGCAAACTCGGAGG5129980.430037958332764No Hit
CCATGCCTACTAGCCTCCCTAACCTTGCCAAGGAGGCAAAGGATGCCAAG5113760.42867826186530067No Hit
GGAAGCATGAGTACTGTGGTAAGTACTAAAACTATTAAGACAAGCCACTA5111030.428449410363687No Hit
TCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTT5005690.41961892788017374No Hit
TCAAAGATTCCAGAAGTCAGGACACGGCAGCGAAGCAGGTCCTGAAACAA4896780.41048917804839635No Hit
CCTATACACTGGAACATTTGCTTCATAAAGATCTTCAAGATTGGGCCACC4728980.39642277439609397No Hit
GCTTCCGAGTCTCCCTTCAGCCCTCCACATCCCTACATTAATCCCTACAT4695230.3935935662717483No Hit
GGTTATCCATGAGCTTGAGATTGATTTTGTAGTCACCCACCTCTAAGGCC4521050.3789923374984586No Hit
CTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCT4420760.37058518837873633No Hit
ATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGAGTGGGTCCCA4330990.3630599139099212No Hit
CTTGAATAAAAGACAATGTACTATGCTCAATTTTTTAAAGTAACTATCTG4317240.36190727356296554No Hit
GGTCTTTCGAATGTATGCAATGTCATCAAAAGATTGTAGTTCTGGCATTC4255440.3567266791308304No Hit
ACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCATAAAAAA4111720.34467886308250567No Hit
GTGATGTAGAATTTCAAGTTTTAAATCACAATGTGAAAAGTGCATAATTT4028870.33773368106466745No Hit
CTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGCTC3994770.3348751330042175No Hit
TCCTTCCCTGTCTAATTGCTTTTTTTTTTTTTGTAGTAATAAAAGAGCAG3990250.33449622868652734No Hit
CTGTCGCAACCGCAATGTGCGGGAAGCCCTGCAGGCCTGGGCGGACACGG3930430.3294816144643538No Hit
AAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGAT3882010.3254226438752925No Hit
GTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCAT3811240.31949010879499795No Hit
ATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTA3613290.30289627921828016No Hit
ACAGTAACTGTGTATCTGTTTAAGTAATCAAAAAGGGATCCATGCTCATG3557260.298199374589922No Hit
CATCTTGCTGAGGGGAAGGCTGGGCAGCTTGTTGCTGGCTGGCAGGCTGG3554000.2979260940422074No Hit
GTTATAGCTGTTATTAAAGAATCCAATTCATCTTTTTTCTCCTTCAGCTT3520140.29508766479508614No Hit
AGGAGAGGGATAGGAAGAGCTCACATGGTGAGGCTCACTCCCCCTCCTCT3433820.28785159826787077No Hit
GCAATCACTACTCCGCATTACTACATCCAAATCCATAAGAAGTTGTTTTT3365390.28211522161753083No Hit
ACTGAGTTGCGATAATGTTTTCTTAATCCGCAATGCTGTAAGCCTAGCTG3360550.2817094922154024No Hit
TGTGCCTACCACGTAACCAAAAATGAAGGAGAACTACTGTTTACAAGCCG3281710.27510046203693095No Hit
AATGACTGGACAAAAGGTGACTTATACTTACAATTTCATTGTAAGTGGGG3251120.2725361516214129No Hit
AAGCAGTCTGTGTCCTGAGGGCCTGAGCAGCCCCCGGCACACTCATCATG3119520.2615043356461865No Hit
TGCCAAGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTTTGTGGGT3110360.26073646760414193No Hit
TGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATGCCAGT3082660.2584144212324567No Hit
AAACCTCCAGACACACCTAGGCCTGTGATGACAGATCAATTTCCCAAATC3063760.25683006468282316No Hit
CACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATCACTGTGGAGGT2965700.24860985287027984No Hit
CCACATGGGTTAATTTGCTTTTATAAAGGCTGCCTACTTTTTTCTCAACT2960190.2481479584476089No Hit
GTCTGTAAGGGCCTCGTGGCTGGGCCTCTGTGCTCCCCATCCCCCCACCC2893910.24259181283333833No Hit
TCGTTTGGCATCATCATTATGCTTGCAAAAACGAACTAGATTTGACAGCC2869360.24053382588659208No Hit
GGATTGACTTCAACTGGGAATACCCTTCTTCGAAGGTAACGCTAATGATT2829530.23719494115791986No Hit
TGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGC2826700.23695770681741918No Hit
CACTATTATCTACCATGAAGTGCGAATAGTGGTGAGAGACAGGAATGACA2720010.22801405600893207No Hit
TCCTGAAGGCTTGCTCTCTTTGGTTAAGGTAATGCCTTGTTGTCCACCTG2718730.22790675567117913No Hit
TCTGCTCACCTCACTGTACCGCAGGGTGAGTGGATGTGGTATTATACCTG2636150.2209842073183357No Hit
ACCAATTCCAGGTGATACAACTCGTTCGTAGTGATATGGATTCACACAGA2576980.21602408154892733No Hit
GCCCAACGCACCGAATAGTTACGGTCGGAGGCCGATCCAGGTGGGTAGAG2506540.21011920983695964No Hit
GCATGTATAGCACCTCGAGTGTTGAGATTACAAGCAGCACATCAGGTAAA2496680.2092926619227064No Hit
TTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAG2465870.20670990926163704No Hit
GCACTTTAGGATAACAAAGTCATACGAATTACATACCTTGAGAAAAGCTC2351590.19713000098163044No Hit
GGAAGATAAACTAAAACGAGAAGAAGAAAAAAGAGGTTGGTTTCTTTTCA2336240.1958432352124836No Hit
GCAAATGCTATCGATTTCTTGATCACATAGACTTCCATTTTCTACTTTTT2322910.19472580278885315No Hit
AGGCACAAGGGGTCTGAGTACCTGTGAGATGGCCCACTGGGCCGCAGCAA2298000.19263763762211386No Hit
ATCAGTGCTTTGGCCATTGCTGCCTTGGCTGAAGCAGCAACTCCTTATGG2035630.1706435832344228No Hit
TCTTGGTGGATGACGGGCCGGAGCCGAGCAGCTGAAGGCACCCGCTGGGT2029910.17016408485008927No Hit
TCCTTCCCTGTCTAATTGCTTTTTTTTTTTTGTAGTAATAAAAGAGCAGT1979130.16590727926329601No Hit
TCATGTCCTCGGAGCAGCAGCACCACCACAGCCACCACCACCACCAGCCC1973030.16539592609119205No Hit
ATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTA1966190.16482253991132464No Hit
CGCACAAGAATCAGGTAGGAAAAAAAAAAAAAGACTCACCAAACTCATCG1952130.16364391276381945No Hit
AGGTACTTTGTTGTTCAGTTTACTCTCAAAATTGCTCATAATGAAAGACA1949460.1634200909655379No Hit
CATGTGTGACTTGATTAGCAGGTCAAAAGTGAACTGATGCAGCTCTCTCG1834450.15377898796165657No Hit
GGATGATTGGAAATGGGAGGCTGGAATGCAAGCTCATTGTGAACAGGCCC1824090.15291052585296852No Hit
CTCCAGTACAAGATGAGGAAAATTACTACAAAGAAAAACAAAAAGCTTCA1772110.14855312619953184No Hit
TCAAAGGCATAGAGACACTGGATATACTGCTTTTTCAAGGAGCTGGCAGC1755140.14713055844041642No Hit
GGGGTAGTTAGGGCAGGAAATGCATTATCTCAGAGGATGGCTCCACAAGA1725260.1446257661809957No Hit
AGCTAAAGGATTAATGGCACCTAAAAATAAAAAGCACTCATTAACACTAA1710720.14340690140683315No Hit
TCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTGTCTGGCTCAGCCTGGA1707740.14315709280800207No Hit
CTGCTGGACCCTGGTGTTCACCTGTTGAGCTGTCTTGGTCTTGTGCTTCT1707210.14311266376190124No Hit
AGGATGAGGACAGTGAAGGCCCAGGGAGTGGAGAGAGTCGCCTACAGCAT1681950.14099515865905762No Hit
ACATTGGAGAGCAGCCCTAGGCAGAAGCGCTCCGAATTGGAGGGGTCGGT1668070.13983162062154894No Hit
CCGTGTCATAGAGCAGCGCATCCCCTACCGTGGCACCCGCTACCCAGAGG1636450.1371809669654953No Hit
TTCTCTGCGTTCAGCTGCACGGTCCCGGTACTTCATTTGCTGAAAGTTTG1548150.12977892022831836No Hit
CGACAAGAGATTGAGAAGGAGAAAAAAGTGGAGCCATGGAAAGAACAATT1531130.12835216104975944No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTAAATAGAG1523190.1276865636421356No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTCCCCCAGAACGGAG1511650.12671918403458154No Hit
GCCAAAGTCCTCGGACACTTCCACTCTCTTTTCAGGAGGCACAAAGATGG1465250.1228295467910367No Hit
CCAGCTTGCCAGATTTTTCCCCATTACACCCGCAGTGTGGCATATCCTTG1460000.12238944774947182No Hit
CTTCCGTGGCAGATTGTGCCGGTATAGACCTTGATCCAAGGGGAGGGCAG1448630.12143631896802559No Hit
AAAACTTGATAGGCAGCTTCAATTTTTTTTCTTTATTTTCAGTAATCTAT1430160.11988800862560589No Hit
CCAGCTTGCCAGATTTTTCCCCATTACACCCCCAGTGTGGCATATCCTTG1428480.11974717693230513No Hit
AGTATATATCCGAGCCGCTGTCCGAAAAGAGAAAGGCCTGCCCATCCTCG1416330.11872866200754069No Hit
TTAAAAATAGGGTTTGGCTGCTTTCTTGAAGGCTATTGGGTATCTTATTC1385570.11615010076591484No Hit
CTCCATTTTAGCACTTACCTGTGATCCATAGAAAATCTTTCTCCTGCGCA1369850.11483231849288628No Hit
ATCCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGCTGCATCC1339530.112290641742363No Hit
GGGCTATCTCACTTCTCAAGAACTAAAACAAAAAGTGACCAATTCTTTTG1314820.11021924225339762No Hit
TTCTTGAGGATCTTGACGGCCCTCCTGCACAGCGTCTCCGAGTCCAGCAC1274270.10682000108473935No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1271800.10661294496423167No Hit
TGAGGGTTTCCAAGACCAGTCTCTGCGGGGAAAGGACGGCATTGGGGCCC1239720.10392373024929809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)