FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007680890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007680890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277762355
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCAC63704602.2934929393149766No Hit
ACTTCTTCTTGCGTTCCTCAGTACTGGGTGGAGCAGCCCCCGGTTTGTCA60222622.168134699174767No Hit
GCTCACTGGAGTATCTGTTTGCTCAGGTAGGAAGTTTGGGCCCAGGAGAA44514071.6025954993073126No Hit
CATATTCATGGAGAGAAGAAGGAGTTCGTGTGCAGGTGGCTGGACTGCTC40844611.4704876044127724No Hit
GCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATGTGTG38573561.3887252648041524No Hit
GTCTGGCTCCGGTAAGTAAGAGTGCCAACCAAAAATGCAAGTGCACCATC34269031.2337535804662947No Hit
ACAAATGTTTCAGAACTTCAAACTATGTGCCCTTCAGAAGTAACAAATAT33508461.206371540160653No Hit
ATGGAAACCTTGCGCCCTCGCTCTAGAGAGCTGGTGTCCATTTCACTGTC33051101.1899056659423846No Hit
GACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGAGACCGG32740811.1787346057027779No Hit
CTTTTGTACAGAGGTGGCAGAAACACTGTAACGCTGGAAAAAGAGAAAGA29405351.0586513784418339No Hit
TGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTA27728930.9982969074408949No Hit
CTTCCGTGGCAGATTGTGCAGGTATAGACCTTGATCCAAGGGGAGGGCAG27050990.9738897123046066No Hit
TTTGGTGAATGGCTGGTGCATAAGTTTGACCTTGTTGAGCTGGCTGGCTT26206530.9434874643109934No Hit
CTCTGTTTTCCTGCTCCTATCAGGTGTGTGTCCTGTATGCAGTGTGGGGC26093960.9394347192944846No Hit
CAGATGCAATGATCCTTGTGGATGGACAGCCACGCCAGGCCAAGGGTGAG25139810.9050834120412034No Hit
TGTCAGAAGAACCCCTGGCCCCCTCCAGCATAGATGCTGAAAGCAATGGA25099890.9036462122449962No Hit
CTCAACAGTCATCAACCACTACCGCATGCGGGGCCATAGCCCCTTTGCCA24243110.8728004196248983No Hit
TATGCTCAGTGGTGTCCCCCCCACCAATCCGCCAGTGCAGCAGGCCACTG23857850.8589302895275353No Hit
GGGAGACCTCTTAGATCAGTTCTTTTGTACTACTTGTGGTCAGCACTATC23532540.8472184792644057No Hit
CCTCCAGCGACTATACTGAATACAGTGAAGAAGAGTCTGAGTTCAGTGAG23136730.832968528078616No Hit
CCTTTCAGTTTCTCTTGTAGTCTCATTAAGACACGTTCAGCTACTTTGTT22981780.8273900183485987No Hit
TCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTT22792470.8205744799362751No Hit
GTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGAGACAACATCAT22597110.8135411294305882No Hit
CATGTGTGACTTGATTAGCAGGTCAAAAGTGAACTGATGCAGCTCTCTCG22376920.8056138492921404No Hit
TCCAAGGAAGGCAGTGACCTGTCAGTGGTGGAGCGTGCAGAAGTCTGGCT22026870.7930113495761513No Hit
CCCTTTCCTCAGAATTACTCAGCTGCGGAGGATCTCCTTTCACGACTTGT21636730.7789655297241413No Hit
ACGTCTTTGGTGTGCTGCAGGCCAAGCAAGGCCAGGAAGCCCTTCTGGTC21290190.7664893970243016No Hit
AAACTTGTGGTAGTTGGAGCTGTTGGCGTAGGCAAGAGTGCCTTGACGAT20860630.7510243783755362No Hit
GTGATGTAGAATTTCAAGTTTTAAATCACAATGTGAAAAGTGCATAATTT20165110.7259842680985334No Hit
TACTTTCCCGTGGCCGACCTGTCTATCATCGCCGCCCTCTATGCCCGCTT20087340.7231843926438484No Hit
CCTATACACTGGAACATTTGCTTCATAAAGATCTTCAAGATTGGGCCACC19961460.7186524610219408No Hit
CCAATAATCTCTTAAAACAGGCCTCTTTTTTTTATGCACAACAACTTCCT19956240.7184645305876672No Hit
GTATCGTCGTCCTCCCCGCAGTCAGGCTGCCCATCACCCACCATTCCAGC19599470.70562009743905No Hit
AGGCACAAGGGGTCTGAGTACCTGTGAGATGGCCCACTGGGCCGCAGCAA19580950.7049533404193667No Hit
CCCAGCCTCCAATTACGCGGACCCCTACCGACAGCTGCAGTATTGTCCCT19177780.6904384145216511No Hit
GGAAGTCATTGAATCCTTTGCCATTAACATGGAAACAGGCTGGATTACAA19048080.6857689552639341No Hit
CTGTGCCCCTAACTGTGTGGCCGAAGTCGTGACATTTGACAAAGAGGACA18796750.6767205728796475No Hit
GTCTGTAAGGGCCTCGTGGCTGGGCCTCTGTGCTCCCCATCCCCCCACCC18566920.6684462334717748No Hit
GCCCAGCACTTGAGGGTACCTGGCACTGCAGCAGGCGAGAGTGGTCCTGA18495410.6658717305302225No Hit
CATCTTTGTGATCATCCTGGCTTCGGTGCTGCGCATCCGGTGCCGCCCCC18479710.6653064991474457No Hit
CCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGA18347940.6605625157519995No Hit
GCCTTTGACGAAGCTCATGCGGATGGTGCACATTCGGGTCAACTGGTAGA17423400.6272772276862356No Hit
ATTCCTCACAGCATTGCCCATGACGCCGAGCCCTGCTCTGACCCTAGGAC17362520.625085425993022No Hit
GTACATGAACTTGTCTTCCCGTCGTGTGGGTCCTGAATTGGAGGAATATA17170200.6181615215640003No Hit
GTTGCCAAGCCAAAGTCTGAGATTTTGAGGTTATCTAAACCAAAAGAAAA17023640.6128850686047791No Hit
ATCCTTATCTCCCTTGCTTGGAGAGCCAGCCCTGTCTGAGCCTGGGGAAC16971920.6110230452215167No Hit
CATGGTGCCGCGCTTTACCGACGAGCAAGGGTCATCTGCAAACTCGGAGG16920650.6091772227377609No Hit
CACATGCCATTTTTCTTCACCAGAATGTTCTTTGACTTTAAGTCTCGATG16745920.6028865934694426No Hit
TCATGTCCTCGGAGCAGCAGCACCACCACAGCCACCACCACCACCAGCCC16481230.5933572243798121No Hit
CAGCCTGAATTATGATCCTTTCCTAGGTACCCCATGGCCTTAGGGAACCT16168990.5821159602423446No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTTAAATAGA16143830.5812101499499455No Hit
GGTTAGAATTTCTAAGAAGTTATCCTGTTTTTTTTCTCTCTTAGATCTGT16040680.5774965437631028No Hit
CCAATTGCCTTCTGGTCATATCTGTTTGTAAGGAAAACTAAAACCACAAA15810320.5692031232958117No Hit
ACATTTTTGTCTTACCTAGTTCATTCATTGCATGTCTATGCTCTTCATCA15220700.5479756247026347No Hit
GGGCTATCTCACTTCTCAAGAACCAAAACAAAAAGTGACCAATTCTTTTG15006470.5402629164776487No Hit
TCAAAGATTCCAGAAGTCAGGACACGGCAGCGAAGCAGGTCCTGAAACAA14991780.5397340471137639No Hit
AGATTTTGAGGGTGCCACCGGTGGTGAGCAGCAGGTTCCCCGGCTTGATG14811340.5332378464317096No Hit
TGCCAAGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTTTGTGGGT14788500.5324155607767654No Hit
TCTTCACTCTGGGCGGCTCCCAAGGGTCATCCTGCTCTGGGGCAGCGCTT14600110.5256331442034324No Hit
TCCTGCAAGCAGCACAAAACCGAGGACCAGCTTTACCTGGCCCGTTGGTA14348310.5165678408796612No Hit
GCAGGGGCATACTAATAGAGTCTATTCATTACAGGTAAGATCTCTTATCT14235200.5124956547837449No Hit
AGAACCATAAGCTCCTCGTCCAATTTCTCCAAGGTCTTTCAAGTCCTCTG14141160.5091100268069084No Hit
GGATTCATCAGCATCGTCAAGCTGGAGAGTCCTCGACGGGCCCCCCGCCC14090660.5072919258623078No Hit
GGACTTCTGGTCGAACATGCCGGCCTGGATCACCTTCTGGTCCACGTTGA14055630.5060307758407362No Hit
TCCTGAAGGCTTGCTCTCTTTGGTTAAGGTAATGCCTTGTTGTCCACCTG13729240.49428008341879154No Hit
CTGCTGGACCCTGGTGTTCACCTGTTGAGCTGTCTTGGTCTTGTGCTTCT13713210.49370297137637675No Hit
CTTTGAAAACGGTCCTGCAAACCGCCACAAGCCATTAAAACCAAAACCTG13648920.491388402866904No Hit
ATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTA13615590.4901884562434676No Hit
ATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGAGTGGGTCCCA13112980.47209349157483926No Hit
CCATGCCTACTAGCCTCCCTAACCTTGCCAAGGAGGCAAAGGATGCCAAG12996180.4678884581029708No Hit
GCCTGTAGTAATCAAGTGTCTCATTTTTTCATTTCTTGTAGCAGAAACTT12903230.4645420723049385No Hit
ATTTGGCCAGTGCTATCCTTGACCTGCCAGTCCTGGAGCACCTCTTTGCC12614180.45413569452203123No Hit
CATCTTGCTGAGGGGAAGGCTGGGCAGCTTGTTGCTGGCTGGCAGGCTGG12154130.4375729749267139No Hit
GCTTCTCAATACCCTGTGTCTCTCGAAGCTTTTTGGCAGCCTCCAAGTTC12075430.43473961761304913No Hit
GGTTATCCATGAGCTTGAGATTGATTTTGTAGTCACCCACCTCTAAGGCC12057110.43408006099314644No Hit
GGAAGCATGAGTACTGTGGTAAGTACTAAAACTATTAAGACAAGCCACTA11976970.43119486080106134No Hit
CACACCTACCTCCCACTGCATTACAGCGAGATGTCATTTCCCAGAGCACC11915000.4289638169290435No Hit
AGTACTTTGGCATAACTTCCTCTTCTAGTTTAGGACCTAGAGACTACTGC11912010.4288561709523236No Hit
CTCTACTGTGTGCCTCTGCAGGCTCGGATGGCGGGTGAGCGAGGAGCCAG11849010.42658804502143566No Hit
CGCTCAGGCATTCCTCCGATCGCACACATTTGTCGTGGCACCAGCCACAC11769240.4237161655689447No Hit
GCCCAACGCACCGAATAGTTACGGTCGGAGGCCGATCCAGGTGGGTAGAG11739930.4226609469810983No Hit
TGGGTGAAATGGCAACTGTTGCCCCAGTTCTCTACACCAATATTAATTTC11473560.4130710945333107No Hit
AAACCTCCAGACACACCTAGGCCTGTGATGACAGATCAATTTCCCAAATC11021470.39679495084926103No Hit
CCAGGATAGTCTTTCTCAGGCTCAGACTTCTCAGCCACCCTCACCGCAAG10938580.39381074516019277No Hit
GGCATCTTACCAGGCAGTCGCTCTCCCGGGGGCCTGTGCAGCCTGCAGCA10874170.39149185641085166No Hit
TTCTCTGCGTTCAGCTGCACGGTCCCGGTACTTCATTTGCTGAAAGTTTG10825000.389721638124792No Hit
GGTCTTTCGAATGTATGCAATGTCATCAAAAGATTGTAGTTCTGGCATTC10614430.3821406972157908No Hit
CCAGCTTGCCAGATTTTTCCCCATTACACCCCCAGTGTGGCATATCCTTG10146020.3652770009096445No Hit
GCGATACACTCTACAGCAGACTGCCAGTCCTTGTAATTTTTATCAAAGTT10063570.36230863610009356No Hit
TGTGCCTACCACGTAACCAAAAATGAAGGAGAACTACTGTTTACAAGCCG9957170.35847802341681617No Hit
ATTGAACTCCAGCTGCCTGAGGTGATGGGGACTTGGAAGGGACACAGGGG9821870.3536069529652425No Hit
GCCAAGAATCAGCAAGCCATGGATAGCTGGGATCACCAGCATTGAGAAGA9737110.35055542353822566No Hit
AGGATGTTGTAATAGGGTGGCTTAGGTGTAACTGGATTTGAAGTGGCTGT9662280.34786139396031546No Hit
AATGACTGGACAAAAGGTGACTTATACTTACAATTTCATTGTAAGTGGGG9558090.34411034569461363No Hit
AGGTACTTTGTTGTTCAGTTTACTCTCAAAATTGCTCATAATGAAAGACA9384530.3378618387650119No Hit
CTCCATTTTAGCACTTACCTGTGATCCATAGAAAATCTTTCTCCTGCGCA9348110.3365506459649653No Hit
CTAATACATTCTTGCTGCCTATGAATAATATAAATATATTTTCTAAGGAA9123380.3284599167514979No Hit
ATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTA9088910.32721892784931206No Hit
GTGCAAAAGAAATGCCAAGAAGGCCGAGTCGGGGAAGAGCGCCAGCACCG8967380.32284360492263253No Hit
GCTTCCGAGTCTCCCTTCAGCCCTCCACATCCCTACATTAATCCCTACAT8683250.31261435697432793No Hit
TGCTGCCGGATGTGGTACTGCTGCTGCTGCTGCTGCTGTTGCTGTTGCTG8355260.3008060613541385No Hit
GGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTTCACAT8167810.2940574866597743No Hit
CTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCT7880740.28372239283469497No Hit
GCAAATGAAAACCAAAACTTAAAAGAACAAAATCAAACAGAGAAATCTTT7843260.28237303791581114No Hit
TGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATGCCAGT7749360.278992450218821No Hit
ACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGA7703550.2773431986490754No Hit
CTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGCTC7570520.27255385273501154No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTCCCCCAGAACGGAG7247880.26093816780895307No Hit
CAGATGGAGGTGATGCTGCAGTTGCTCATGCAGGATTTCTGGTTGTCACA7157340.257678546828277No Hit
AGGAGAGGGATAGGAAGAGCTCACATGGTGAGGCTCACTCCCCCTCCTCT7065560.2543742833689612No Hit
ATCAGTGCTTTGGCCATTGCTGCCTTGGCTGAAGCAGCAACTCCTTATGG6977860.2512169080651696No Hit
GCAAGTGAGGTTAGAAAGAATTCCAGCTGCACAGGTGACCACATTTATAT6849940.24661153236549999No Hit
ACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCATAAAAAA6807580.2450864876919696No Hit
GGATTGACTTCAACTGGGAATACCCTTCTTCGAAGGTAACGCTAATGATT6667070.24002784682611147No Hit
AGGATGAGGACAGTGAAGGCCCAGGGAGTGGAGAGAGTCGCCTACAGCAT6657420.2396804275366977No Hit
GGGGTAGTTAGGGCAGGAAATGCATTATCTCAGAGGATGGCTCCACAAGA6573280.23665121934900069No Hit
CACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATCACTGTGGAGGT6560390.23618715358314124No Hit
TCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTGTCTGGCTCAGCCTGGA6508340.23431324954024096No Hit
TTAAAAATAGGGTTTGGCTGCTTTCTTGAAGGCTATTGGGTATCTTATTC6318330.22747250972868518No Hit
CTGTCGCAACCGCAATGTGCGGGAAGCCCTGCAGGCCTGGGCGGACACGG6191470.2229052961478527No Hit
TGAGGGTTTCCAAGACCAGTCTCTGCGGGGAAAGGACGGCATTGGGGCCC6099880.2196078730683285No Hit
TTCTTGAGGATCTTGACGGCCCTCCTGCACAGCGTCTCCGAGTCCAGCAC6027100.21698764758816938No Hit
ACAGTAACTGTGTATCTGTTTAAGTAATCAAAAAGGGATCCATGCTCATG5967730.21485020891329926No Hit
GTTATAGCTGTTATTAAAGAATCCAATTCATCTTTTTTCTCCTTCAGCTT5928350.2134324501964998No Hit
TCGTTTGGCATCATCATTATGCTTGCAAAAACGAACTAGATTTGACAGCC5914900.21294822331125468No Hit
TCTTGGTGGATGACGGGCCGGAGCCGAGCAGCTGAAGGCACCCGCTGGGT5550740.19983773539074437No Hit
GCACTTTAGGATAACAAAGTCATACGAATTACATACCTTGAGAAAAGCTC5492520.1977416990146127No Hit
GCATGTATAGCACCTCGAGTGTTGAGATTACAAGCAGCACATCAGGTAAA5482870.1973942797251989No Hit
CCGTGTCATAGAGCAGCGCATCCCCTACCGTGGCACCCGCTACCCAGAGG5237290.18855290883460432No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTTCCCCCAGAACGGA5120760.1843575959024397No Hit
AAGCAGTCTGTGTCCTGAGGGCCTGAGCAGCCCCCGGCACACTCATCATG5017260.18063138901598094No Hit
CACTATTATCTACCATGAAGTGCGAATAGTGGTGAGAGACAGGAATGACA5014840.18054426417863573No Hit
TCAAAGGCATAGAGACACTGGATATACTGCTTTTTCAAGGAGCTGGCAGC4980850.17932055623592333No Hit
TTGGGTAATCCGGTCCCCAGCCTTTCACAAAACTCATCCTGAGTATGCAT4885280.17587984520076524No Hit
TGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGC4868680.17528221201897573No Hit
GTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCAT4763620.1714998420142283No Hit
ACATTGGAGAGCAGCCCTAGGCAGAAGCGCTCCGAATTGGAGGGGTCGGT4701220.16925331728268217No Hit
GCCAAAGTCCTCGGACACTTCCACTCTCTTTTCAGGAGGCACAAAGATGG4427010.15938120916349519No Hit
AGCTAAAGGATTAATGGCACCTAAAAATAAAAAGCACTCATTAACACTAA4417930.15905431101345607No Hit
ACTGAGTTGCGATAATGTTTTCTTAATCCGCAATGCTGTAAGCCTAGCTG4317710.15544619068339913No Hit
ATCCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGCTGCATCC4289510.15443093431433502No Hit
CCACAGATTTTGCATCCTTAGCAACCCCTGCTTGTTCTGACTCCTGTTTT4249190.15297933371856673No Hit
TCTGCTCACCTCACTGTACCGCAGGGTGAGTGGATGTGGTATTATACCTG4060540.14618755662551897No Hit
TTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAG4039880.14544375532818335No Hit
TCAACAAGATGTTTTGCCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGG3974640.1430949849197527No Hit
CGCACAAGAATCAGGTAGGAAAAAAAAAAAAAGACTCACCAAACTCATCG3915660.14097158702445475No Hit
AGGTCCAGTTCCCATCATGCCCCCTTGATTCATATTGGGGTAGCCTGGCG3901040.14044523780049317No Hit
GTACAGATGTATTTGGACTCATTCAATATTGCCCCGTTATAGAACCACTG3842940.13835352166422984No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTAAATAGAG3499820.12600051580063829No Hit
TCCTTCCCTGTCTAATTGCTTTTTTTTTTTTTGTAGTAATAAAAGAGCAG3448370.1241482129570798No Hit
GCAATCACTACTCCGCATTACTACATCCAAATCCATAAGAAGTTGTTTTT3420390.12314087702777433No Hit
GGAAGATAAACTAAAACGAGAAGAAGAAAAAAGAGGTTGGTTTCTTTTCA3267270.11762825095574957No Hit
GCAAATGCTATCGATTTCTTGATCACATAGACTTCCATTTTCTACTTTTT3220380.1159401172271887No Hit
AGTGCTTGAGGTCCTCGCCGCACAGCCGGCACACGGTGTCCGGGGCGCAG3171140.1141673788012058No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2987660.10756173204248647No Hit
CGACAAGAGATTGAGAAGGAGAAAAAAGTGGAGCCATGGAAAGAACAATT2926960.10537641070907539No Hit
CGTTCCACTGCCAATTTATACTGGCAGGCTAGGAAAAAAAAAAACAGTTA2789940.10044341681938866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)