FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007680904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007680904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341208354
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGAGACCGG58005141.6999917885949531No Hit
GCTCACTGGAGTATCTGTTTGCTCAGGTAGGAAGTTTGGGCCCAGGAGAA55388651.6233087305945624No Hit
CCAATAATCTCTTAAAACAGGCCTCTTTTTTTTATGCACAACAACTTCCT53904371.5798080371736738No Hit
TTGGGTAATCCGGTCCCCAGCCTTTCACAAAACTCATCCTGAGTATGCAT51873991.5203024601208914No Hit
TGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTA51420691.507017322324998No Hit
CCCTTTCCTCAGAATTACTCAGCTGCGGAGGATCTCCTTTCACGACTTGT48418111.4190188907273942No Hit
CTTTTGTACAGAGGTGGCAGAAACACTGTAACGCTGGAAAAAGAGAAAGA48388391.4181478686773303No Hit
CATATTCATGGAGAGAAGAAGGAGTTCGTGTGCAGGTGGCTGGACTGCTC46167751.3530662265086277No Hit
AGGATGTTGTAATAGGGTGGCTTAGGTGTAACTGGATTTGAAGTGGCTGT45764871.3412587782068197No Hit
TGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCAC45324221.328344381626717No Hit
ACAAATGTTTCAGAACTTCAAACTATGTGCCCTTCAGAAGTAACAAATAT43717131.281244421114027No Hit
GTTGCCAAGCCAAAGTCTGAGATTTTGAGGTTATCTAAACCAAAAGAAAA41743141.2233914999630988No Hit
AGAACCATAAGCTCCTCGTCCAATTTCTCCAAGGTCTTTCAAGTCCTCTG39983281.1718142164831051No Hit
GTATCGTCGTCCTCCCCGCAGTCAGGCTGCCCATCACCCACCATTCCAGC39799711.1664342192512671No Hit
GTCTGGCTCCGGTAAGTAAGAGTGCCAACCAAAAATGCAAGTGCACCATC38609231.1315441004706468No Hit
CCCAGCCTCCAATTACGCGGACCCCTACCGACAGCTGCAGTATTGTCCCT37175741.0895319403580606No Hit
GCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATGTGTG35160401.0304671496993887No Hit
TTTGGTGAATGGCTGGTGCATAAGTTTGACCTTGTTGAGCTGGCTGGCTT33392060.9786413377205881No Hit
CTGTGCCCCTAACTGTGTGGCCGAAGTCGTGACATTTGACAAAGAGGACA33003610.9672567981732358No Hit
CTCAACAGTCATCAACCACTACCGCATGCGGGGCCATAGCCCCTTTGCCA29841880.8745940610821035No Hit
ATGGAAACCTTGCGCCCTCGCTCTAGAGAGCTGGTGTCCATTTCACTGTC28235830.8275245804796444No Hit
TGTCAGAAGAACCCCTGGCCCCCTCCAGCATAGATGCTGAAAGCAATGGA27430490.8039219930705449No Hit
CCTTTCAGTTTCTCTTGTAGTCTCATTAAGACACGTTCAGCTACTTTGTT26879540.7877749675495929No Hit
GTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGAGACAACATCAT26449270.7751647839196809No Hit
GGTTAGAATTTCTAAGAAGTTATCCTGTTTTTTTTCTCTCTTAGATCTGT25941090.7602712447069804No Hit
GTACATGAACTTGTCTTCCCGTCGTGTGGGTCCTGAATTGGAGGAATATA25474450.7465951434471619No Hit
CTTCCGTGGCAGATTGTGCAGGTATAGACCTTGATCCAAGGGGAGGGCAG25377710.7437599256435556No Hit
GCCTGTAGTAATCAAGTGTCTCATTTTTTCATTTCTTGTAGCAGAAACTT25301630.7415302029797313No Hit
GCAGGGGCATACTAATAGAGTCTATTCATTACAGGTAAGATCTCTTATCT24997490.7326165876935123No Hit
CTCTGTTTTCCTGCTCCTATCAGGTGTGTGTCCTGTATGCAGTGTGGGGC24914780.7301925556019652No Hit
TCCAAGGAAGGCAGTGACCTGTCAGTGGTGGAGCGTGCAGAAGTCTGGCT24901760.7298109705719573No Hit
TATGCTCAGTGGTGTCCCCCCCACCAATCCGCCAGTGCAGCAGGCCACTG23554640.6903301083888468No Hit
TACTTTCCCGTGGCCGACCTGTCTATCATCGCCGCCCTCTATGCCCGCTT23547110.6901094221157317No Hit
TGGGTGAAATGGCAACTGTTGCCCCAGTTCTCTACACCAATATTAATTTC23304280.6829926561528443No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTTAAATAGA23230780.6808385471124778No Hit
CTTTGAAAACGGTCCTGCAAACCGCCACAAGCCATTAAAACCAAAACCTG21566180.6320531061792233No Hit
CACATGCCATTTTTCTTCACCAGAATGTTCTTTGACTTTAAGTCTCGATG21413230.6275705078428414No Hit
GGAAGTCATTGAATCCTTTGCCATTAACATGGAAACAGGCTGGATTACAA21180740.620756782525905No Hit
GGGCTATCTCACTTCTCAAGAACCAAAACAAAAAGTGACCAATTCTTTTG20939340.6136819264395853No Hit
GGGAGACCTCTTAGATCAGTTCTTTTGTACTACTTGTGGTCAGCACTATC20838500.6107265474514144No Hit
GCCAAGAATCAGCAAGCCATGGATAGCTGGGATCACCAGCATTGAGAAGA20597420.603661069799012No Hit
ACTTCTTCTTGCGTTCCTCAGTACTGGGTGGAGCAGCCCCCGGTTTGTCA20577880.6030883991779404No Hit
GCCCAGCACTTGAGGGTACCTGGCACTGCAGCAGGCGAGAGTGGTCCTGA20256810.5936786061222874No Hit
CATCTTTGTGATCATCCTGGCTTCGGTGCTGCGCATCCGGTGCCGCCCCC20136660.5901572972624228No Hit
CACACCTACCTCCCACTGCATTACAGCGAGATGTCATTTCCCAGAGCACC19924340.5839347063583326No Hit
ATCCTTATCTCCCTTGCTTGGAGAGCCAGCCCTGTCTGAGCCTGGGGAAC19337050.5667226424356538No Hit
ACGTCTTTGGTGTGCTGCAGGCCAAGCAAGGCCAGGAAGCCCTTCTGGTC18801720.5510334017202874No Hit
CAGATGCAATGATCCTTGTGGATGGACAGCCACGCCAGGCCAAGGGTGAG18698120.5479971337395801No Hit
CCAATTGCCTTCTGGTCATATCTGTTTGTAAGGAAAACTAAAACCACAAA18619210.5456844705508002No Hit
CCTCCAGCGACTATACTGAATACAGTGAAGAAGAGTCTGAGTTCAGTGAG18596870.5450297386329527No Hit
CCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGA17956340.5262573377672928No Hit
ATTCCTCACAGCATTGCCCATGACGCCGAGCCCTGCTCTGACCCTAGGAC17625140.516550658662947No Hit
TCAACAAGATGTTTTGCCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGG17594160.5156427090293341No Hit
ACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGA17534380.5138907003431692No Hit
CTTGAATAAAAGACAATGTACTATGCTCAATTTTTTAAAGTAACTATCTG17502350.5129519777232652No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTTTCCCCCAGAACGG17372930.5091589873558606No Hit
TGCCAAGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTTTGTGGGT16529760.484447693212107No Hit
GGACTTCTGGTCGAACATGCCGGCCTGGATCACCTTCTGGTCCACGTTGA16490770.48330498965450297No Hit
TCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTT16123530.47254206443022784No Hit
CGCTCAGGCATTCCTCCGATCGCACACATTTGTCGTGGCACCAGCCACAC16098990.47182285577919936No Hit
GCTTCCGAGTCTCCCTTCAGCCCTCCACATCCCTACATTAATCCCTACAT16079630.47125546052720624No Hit
GGCATCTTACCAGGCAGTCGCTCTCCCGGGGGCCTGTGCAGCCTGCAGCA15744600.4614365332919135No Hit
ACATTTTTGTCTTACCTAGTTCATTCATTGCATGTCTATGCTCTTCATCA15739200.46127827221956No Hit
GCTTCTCAATACCCTGTGTCTCTCGAAGCTTTTTGGCAGCCTCCAAGTTC15637320.458292413321158No Hit
CATGGTGCCGCGCTTTACCGACGAGCAAGGGTCATCTGCAAACTCGGAGG15502380.45433764496868095No Hit
TCCTGCAAGCAGCACAAAACCGAGGACCAGCTTTACCTGGCCCGTTGGTA15382550.45082571454273357No Hit
CTTGTGCAGTAGCCGCCTGCTGCTGCATCTGTCGATGACACTGACGCAAA15372820.4505405515364375No Hit
GCCTTTGACGAAGCTCATGCGGATGGTGCACATTCGGGTCAACTGGTAGA14917180.43718683394252417No Hit
GGATTCATCAGCATCGTCAAGCTGGAGAGTCCTCGACGGGCCCCCCGCCC14794610.43359460067616046No Hit
ATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGAGTGGGTCCCA14565560.4268816935238344No Hit
ATTTGGCCAGTGCTATCCTTGACCTGCCAGTCCTGGAGCACCTCTTTGCC14394800.421877126724746No Hit
GGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTTCACAT14294290.4189314192465522No Hit
CCTATACACTGGAACATTTGCTTCATAAAGATCTTCAAGATTGGGCCACC14269730.4182116244434039No Hit
CTAATACATTCTTGCTGCCTATGAATAATATAAATATATTTTCTAAGGAA14260950.41795430366279956No Hit
GCGATACACTCTACAGCAGACTGCCAGTCCTTGTAATTTTTATCAAAGTT14204230.4162919762509683No Hit
ATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTA14035170.4113372323820653No Hit
ACAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCATAAAAAA13735210.40254612288889036No Hit
CCATGCCTACTAGCCTCCCTAACCTTGCCAAGGAGGCAAAGGATGCCAAG13622170.3992331911076245No Hit
CAGATGGAGGTGATGCTGCAGTTGCTCATGCAGGATTTCTGGTTGTCACA13474940.3949182322775134No Hit
CTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGCTC13426100.39348684880089424No Hit
GGTTATCCATGAGCTTGAGATTGATTTTGTAGTCACCCACCTCTAAGGCC13387400.3923526444490278No Hit
GCAAGTGAGGTTAGAAAGAATTCCAGCTGCACAGGTGACCACATTTATAT13240260.3880403233034558No Hit
GGAAGCATGAGTACTGTGGTAAGTACTAAAACTATTAAGACAAGCCACTA13231640.3877876917398101No Hit
AAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGAT13187280.38648760633803236No Hit
CCTCCAGCGACTATACTGAATACAGTGAAGAAGCGTCTGAGTTCAGTGAG13040320.3821805605615389No Hit
GGTCTTTCGAATGTATGCAATGTCATCAAAAGATTGTAGTTCTGGCATTC12851870.37665754221246295No Hit
CATCTTGCTGAGGGGAAGGCTGGGCAGCTTGTTGCTGGCTGGCAGGCTGG12515630.36680315277392067No Hit
GCAAATGAAAACCAAAACTTAAAAGAACAAAATCAAACAGAGAAATCTTT12428610.36425280490055056No Hit
GTGCAAAAGAAATGCCAAGAAGGCCGAGTCGGGGAAGAGCGCCAGCACCG12409820.36370211498397254No Hit
CAGCCTGAATTATGATCCTTTCCTAGGTACCCCATGGCCTTAGGGAACCT11851810.34734817776472143No Hit
GCAATCACTACTCCGCATTACTACATCCAAATCCATAAGAAGTTGTTTTT11671840.3420736879144524No Hit
CTCTACTGTGTGCCTCTGCAGGCTCGGATGGCGGGTGAGCGAGGAGCCAG11446310.335463943535216No Hit
CCAGGATAGTCTTTCTCAGGCTCAGACTTCTCAGCCACCCTCACCGCAAG11293680.3309907236327514No Hit
CCACATGGGTTAATTTGCTTTTATAAAGGCTGCCTACTTTTTTCTCAACT11186520.3278501205747149No Hit
TGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATGCCAGT11151470.32682288898471695No Hit
TCTTCACTCTGGGCGGCTCCCAAGGGTCATCCTGCTCTGGGGCAGCGCTT11149910.3267771691193704No Hit
AAACCTCCAGACACACCTAGGCCTGTGATGACAGATCAATTTCCCAAATC10646180.31201404875333155No Hit
TGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGC10418740.3053483268466516No Hit
CACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATCACTGTGGAGGT10196600.298837935251726No Hit
CCAGCTTGCCAGATTTTTCCCCATTACACCCCCAGTGTGGCATATCCTTG10151700.2975220237427129No Hit
GTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCAT10091370.29575389587325285No Hit
GTTATAGCTGTTATTAAAGAATCCAATTCATCTTTTTTCTCCTTCAGCTT10051140.29457485088421953No Hit
TCAAAGATTCCAGAAGTCAGGACACGGCAGCGAAGCAGGTCCTGAAACAA9833450.2881948781359556No Hit
GGATGATTGGAAATGGGAGGCTGGAATGCAAGCTCATTGTGAACAGGCCC9700850.28430868958149835No Hit
GTGATGTAGAATTTCAAGTTTTAAATCACAATGTGAAAAGTGCATAATTT9607370.2815690145734239No Hit
AATGACTGGACAAAAGGTGACTTATACTTACAATTTCATTGTAAGTGGGG8958900.26256391131619244No Hit
CATGTGTGACTTGATTAGCAGGTCAAAAGTGAACTGATGCAGCTCTCTCG8944510.26214217486597646No Hit
AGATTTTGAGGGTGCCACCGGTGGTGAGCAGCAGGTTCCCCGGCTTGATG8818390.25844589959834335No Hit
ACTGAGTTGCGATAATGTTTTCTTAATCCGCAATGCTGTAAGCCTAGCTG8636600.25311806990517005No Hit
GCCCAACGCACCGAATAGTTACGGTCGGAGGCCGATCCAGGTGGGTAGAG8373630.2454110487576163No Hit
TGTGCCTACCACGTAACCAAAAATGAAGGAGAACTACTGTTTACAAGCCG8263040.24216992061102935No Hit
ACAGTAACTGTGTATCTGTTTAAGTAATCAAAAAGGGATCCATGCTCATG8230010.24120189038513398No Hit
ACCAATTCCAGGTGATACAACTCGTTCGTAGTGATATGGATTCACACAGA8166210.23933206512288382No Hit
TCGTTTGGCATCATCATTATGCTTGCAAAAACGAACTAGATTTGACAGCC7953990.23311240497939273No Hit
GCATGTATAGCACCTCGAGTGTTGAGATTACAAGCAGCACATCAGGTAAA7838460.22972649725920835No Hit
TCATGTCCTCGGAGCAGCAGCACCACCACAGCCACCACCACCACCAGCCC7715760.2261304540040658No Hit
AGGTACTTTGTTGTTCAGTTTACTCTCAAAATTGCTCATAATGAAAGACA7628380.22356955539253884No Hit
CACTATTATCTACCATGAAGTGCGAATAGTGGTGAGAGACAGGAATGACA7536750.2208840994555485No Hit
AGCTAAAGGATTAATGGCACCTAAAAATAAAAAGCACTCATTAACACTAA7476820.21912769462848497No Hit
CTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCT7465420.21879358792018322No Hit
AGGCACAAGGGGTCTGAGTACCTGTGAGATGGCCCACTGGGCCGCAGCAA7357330.21562572878857472No Hit
AGGATGAGGACAGTGAAGGCCCAGGGAGTGGAGAGAGTCGCCTACAGCAT7248200.2124273897467352No Hit
GGATTGACTTCAACTGGGAATACCCTTCTTCGAAGGTAACGCTAATGATT6932170.20316530702527874No Hit
GCACTTTAGGATAACAAAGTCATACGAATTACATACCTTGAGAAAAGCTC6920440.20282152880699983No Hit
GGGGTAGTTAGGGCAGGAAATGCATTATCTCAGAGGATGGCTCCACAAGA6895790.20209909631931228No Hit
AGTACTTTGGCATAACTTCCTCTTCTAGTTTAGGACCTAGAGACTACTGC6608820.19368869262796537No Hit
TTAAAAATAGGGTTTGGCTGCTTTCTTGAAGGCTATTGGGTATCTTATTC6490680.1902262920561435No Hit
TCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTGTCTGGCTCAGCCTGGA6363400.1864960199655604No Hit
GTCTGTAAGGGCCTCGTGGCTGGGCCTCTGTGCTCCCCATCCCCCCACCC6208510.18195656487355524No Hit
TCAAAGGCATAGAGACACTGGATATACTGCTTTTTCAAGGAGCTGGCAGC6122700.17944167920343473No Hit
GCAAATGCTATCGATTTCTTGATCACATAGACTTCCATTTTCTACTTTTT6080730.17821163897997644No Hit
ATCAGTGCTTTGGCCATTGCTGCCTTGGCTGAAGCAGCAACTCCTTATGG6057870.17754166710701344No Hit
GGTAGCATTAGACTCAGATGGGGCTAACAGAGCTGGGGTGCTGTATGTCT5911220.17324370668837727No Hit
TCTTGGTGGATGACGGGCCGGAGCCGAGCAGCTGAAGGCACCCGCTGGGT5877150.17224519655225087No Hit
GGAAGATAAACTAAAACGAGAAGAAGAAAAAAGAGGTTGGTTTCTTTTCA5770850.16912979803536698No Hit
TTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAG5714210.1674698152320151No Hit
ATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTA5629040.1649736864297291No Hit
CGCACAAGAATCAGGTAGGAAAAAAAAAAAAAGACTCACCAAACTCATCG5619600.16469702262917046No Hit
AGGAGAGGGATAGGAAGAGCTCACATGGTGAGGCTCACTCCCCCTCCTCT5436360.1593266969073096No Hit
TGAGGGTTTCCAAGACCAGTCTCTGCGGGGAAAGGACGGCATTGGGGCCC5388790.15793253409029956No Hit
GATGCATTTTAAAATAAAGACTCTTTTCTTCCTTTTTTTTTTAAATAGAG5334260.1563343903355895No Hit
TCCTGAAGGCTTGCTCTCTTTGGTTAAGGTAATGCCTTGTTGTCCACCTG5309920.15562104320575926No Hit
CTCTGTTTTCCTGCTCCTATCAGTTGTGTGTCCTGTATGCAGTGTGGGGC5268020.15439305451472035No Hit
GAAACAATTAAACAAACGATCTTGTGATTTTTTTTTTCCCCCAGAACGGA5249590.1538529153362992No Hit
CCGTGTCATAGAGCAGCGCATCCCCTACCGTGGCACCCGCTACCCAGAGG5236920.15348158796838837No Hit
TCCTTCCCTGTCTAATTGCTTTTTTTTTTTTTGTAGTAATAAAAGAGCAG5208960.15266214730486932No Hit
TCCTTCCCTGTCTAATTGCTTTTTTTTTTTTTATAGTAATAAAAGAGCAG5153210.1510282482708498No Hit
AAGCAGTCTGTGTCCTGAGGGCCTGAGCAGCCCCCGGCACACTCATCATG4973090.14574936227968205No Hit
CTCCAGTACAAGATGAGGAAAATTACTACAAAGAAAAACAAAAAGCTTCA4953330.14517024398529235No Hit
AGGTCCAGTTCCCATCATGCCCCCTTGATTCATATTGGGGTAGCCTGGCG4474840.13114684759447595No Hit
CTGCTGGACCCTGGTGTTCACCTGTTGAGCTGTCTTGGTCTTGTGCTTCT4284320.12556316250099786No Hit
CGACAAGAGATTGAGAAGGAGAAAAAAGTGGAGCCATGGAAAGAACAATT4251100.12458956382996414No Hit
ACATTGGAGAGCAGCCCTAGGCAGAAGCGCTCCGAATTGGAGGGGTCGGT4124240.12087160093389741No Hit
CTCCATTTTAGCACTTACCTGTGATCCATAGAAAATCTTTCTCCTGCGCA3828540.11220534184224575No Hit
CTGTCGCAACCGCAATGTGCGGGAAGCCCTGCAGGCCTGGGCGGACACGG3781530.11082759128459088No Hit
TTCTCTGCGTTCAGCTGCACGGTCCCGGTACTTCATTTGCTGAAAGTTTG3704270.1085632856456967No Hit
GCCAAAGTCCTCGGACACTTCCACTCTCTTTTCAGGAGGCACAAAGATGG3638540.10663689670388317No Hit
AGTATATATCCGAGCCGCTGTCCGAAAAGAGAAAGGCCTGCCCATCCTCG3635840.10655776616770643No Hit
ATTGAACTCCAGCTGCCTGAGGTGATGGGGACTTGGAAGGGACACAGGGG3594520.10534677588814254No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3525440.10332220646625785No Hit
TTCTACGACGGCATCTGCAAATGGGAGGAGGGCAGTCCCACGCCTGTGCT3434400.10065404201680243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)