FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007698609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29627390
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGCTC885638229.89254875302887No Hit
TCGATACT867559029.282329628090764No Hit
AGATCTGG724402624.450435897323388No Hit
GTTGAGAA444607615.006640814462562No Hit
CACCGCTA626090.211321348252411No Hit
AGATCTGA390210.1317058303144489Illumina PCR Primer Index 9 (100% over 8bp)
TCGATACA368210.12428026903483567No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)