FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75203957
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG806272910.721149952255837TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT25475653.387541163558721TruSeq Adapter, Index 7 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12032001.5999158129405344No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG7000660.9308898466605953No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT5645880.7507424110675452No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC5609590.7459168671137877No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC4852570.6452546107381025No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG4216750.5607085276111203No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC4111360.5466946373579784No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA3577510.4757076811795954No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTAACTCGTATG2411700.32068791273842145TruSeq Adapter, Index 7 (97% over 34bp)
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT2409230.3203594725740296No Hit
CTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA2365780.3145818510587149No Hit
ACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTG2348370.3122668133007948No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG2134390.28381352326979287No Hit
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGA2049680.27254948832014253No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT1991040.264752026279681No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA1957200.26025226305578575No Hit
GCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT1873750.24915577248149323No Hit
GCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCTGG1871760.24889115874580908No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA1811260.24084636929410508No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG1765340.23474030761439854No Hit
CTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC1747450.2323614434277707No Hit
GCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTT1652010.21967062185304959No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG1640300.21811352293603378No Hit
CTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAACAC1320070.17553198696712197No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTAGCTCGTATG1218600.16203934588175992TruSeq Adapter, Index 7 (97% over 34bp)
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT1164900.15489876417008216No Hit
TACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCT1138040.15132714359697855No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT1123170.14934985402430354No Hit
CGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTC1053600.14009901101347633No Hit
TGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCTG1045450.13901529144270958No Hit
GTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTG993200.13206751873441977No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC963410.12810629100274604No Hit
TGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATT932940.12405464249706968No Hit
CCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCC926390.12318367768866204No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTACCTCGTATG909330.120915180035008TruSeq Adapter, Index 7 (97% over 34bp)
CTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTA873120.1161002738193683No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG868380.11546998783587943No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA795750.10581225134204042No Hit
TCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAAC768940.10224727935526053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)