FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93110483
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC5180030.5563315572103734Illumina Single End PCR Primer 1 (96% over 31bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1585190.1702482845030457No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1221090.13114420209806021No Hit
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC1038910.11157819898754043No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC1033500.11099716881503022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)