FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95239359
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC7943250.8340301828364889Illumina Single End PCR Primer 1 (96% over 33bp)
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG2075070.2178794588485208No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT1982890.20820068728098015Illumina Single End PCR Primer 1 (97% over 34bp)
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT967100.10154415255986761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)