FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99989817
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC7014650.701536437455426Illumina Single End PCR Primer 1 (96% over 31bp)
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG5763650.5764236972250885No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT2714600.2714876455869501No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC2155090.2155309475163856No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA1651590.1651758198537357No Hit
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC1522820.152297508455286No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1507940.15080935691681485No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT1492480.1492631994716022Illumina Single End PCR Primer 1 (96% over 32bp)
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA1478130.14782805333067064No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA1456970.14571183783644687No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA1262410.1262538564302003No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG1242840.12429665712859539No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC1212380.12125034692282714No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC1186950.11870708794276522No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA1150950.11510672131743176No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG1075400.10755095191343335No Hit
CCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGAT1020000.10201038771778131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)