FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92213788
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC18551222.011762058836581Illumina Single End PCR Primer 1 (96% over 33bp)
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC7243790.7855430469898927No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT2872540.31150873012612823Illumina Single End PCR Primer 1 (97% over 34bp)
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG2660970.28856530652444295No Hit
CGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCT2400210.26028753964645723No Hit
CCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGG2317790.2513496137909441No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1715370.1860209885315632No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT1643270.17820220117191152No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC1633230.17711342689880608No Hit
CGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCG1452150.15747645026793608No Hit
GGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTC1288230.13970036671739372No Hit
CGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCT1241930.13467942559739549No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1139370.12355744457650954No Hit
CTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACA1053550.11425081030181734No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC1031090.11181516586218104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA961810.10430218960314265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)