FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97728359
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG5016170.513276806377154TruSeq Adapter, Index 1 (97% over 35bp)
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT2441260.24980057221670937No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG2144700.2194552350971124No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT1776690.18179881645203927No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC1473280.1507525568908816No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT1302660.133293960251599No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1269040.12985381244353034TruSeq Adapter, Index 1 (97% over 36bp)
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG1178130.12055149723735768No Hit
CTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC1147530.11742036925023985No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT1136250.11626614952165522No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1116010.11419510277462042No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTG1114520.11404263935302546No Hit
CCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGT1086800.11120620576469517No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA1085870.11111104403175336No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA1060050.10846902688706765No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1058610.10832167968767388No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGA1044040.10683081253825208No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC1000500.10237560624547068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)