FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88930693
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG7984070.8977856497756067TruSeq Adapter, Index 2 (97% over 36bp)
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC1517260.1706115120456781No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1269100.14270663560442512TruSeq Adapter, Index 2 (97% over 37bp)
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC1250560.14062186606372223No Hit
CGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC899020.10109220671427806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)