FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90978540
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC12509821.3750297597653247Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT10875501.1953917923941184Illumina Single End PCR Primer 1 (97% over 34bp)
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC2558620.28123335459109366No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG2207900.24268360428734073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1966620.21616306438859098No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT1011440.11117347013922185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)