FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75869218
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC50289776.628481395445515Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT30733174.050808853730376Illumina Single End PCR Primer 1 (97% over 34bp)
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC13838891.8240454251156244No Hit
CCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACG9484761.2501460078315292No Hit
GGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGT9029981.1902033839336528No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGC8926441.1765562154601357No Hit
CACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAA8876361.1699553829591336No Hit
CCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGG7035160.927274616169103No Hit
CCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGG6955560.9167828776092037No Hit
CGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTC6579230.8671804156463033No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG6200160.8172168058988034No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAA5376300.7086273118038465No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAG5051710.6658444799048805No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC4680680.6169405884742347No Hit
GCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTT4529310.5969891504615218No Hit
GTGCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCG4389320.5785376620067443No Hit
CACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGG4228370.557323524805541No Hit
GGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTT4127300.5440019165612067No Hit
CGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCT4022970.5302506215366554No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC3962710.5223080063906813No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG3883130.5118189039459983No Hit
CACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAG3857930.5084973987737688No Hit
CCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCT3787410.4992024565219586No Hit
GGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCC3449260.4546323385065073No Hit
CCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAG3408920.44931529411572424No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC3388790.4466620441507648No Hit
CCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCC3306940.43587374262905937No Hit
GGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTC3289550.4335816404486995No Hit
CCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGA3265500.43041171190139327No Hit
CGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCT3140520.41393862791626507No Hit
GCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTC3069220.4045408771710287No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT3001900.3956677133537873No Hit
GGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGA2939960.3875036645296647No Hit
CCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGA2838030.37406870333104No Hit
CGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCG2810690.3704651338306927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2754350.36303919726706557No Hit
CCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGA2695980.35534569500900876No Hit
CCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGG2618450.34512679437397126No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG2609980.3440103995799719No Hit
GGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCG2573070.3391454489487423No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC2534670.3340841077339165No Hit
CCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGAC2462460.3245664137463497No Hit
CGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTC2440760.3217062287369299No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC2331610.3073196299453093No Hit
ACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGA2307720.3041707903197315No Hit
GGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTC2200900.29009129895078134No Hit
GTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGC2130810.28085303317611626No Hit
CCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGG2071470.2730316793300809No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGAT2050040.2702070818760779No Hit
CCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGG2038240.26865177389860534No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT2008290.2647041913625629No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA2001930.26386590672385735No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTT1942200.2559931486311089No Hit
GCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCG1934930.25503492075007284No Hit
AGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACCGAAGA1901140.2505812040925478No Hit
CCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGC1883480.24825351435677118No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1875610.24721620301925346No Hit
CCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACG1872030.2467443383955796No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC1819830.23986407768167586No Hit
GCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCG1762090.23225361305292486No Hit
GCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGAC1757310.23162358151628767No Hit
CCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCAC1721190.22686275743609222No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1709510.22532326614991602No Hit
GCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCC1672590.22045699746107836No Hit
CGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGC1672310.22042009184805358No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG1668840.21996272585806803No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1643890.21667417212603932No Hit
CGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGG1626420.21437152548481522No Hit
GGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCTC1568210.20669911214848688No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCACTGTTAGAAGT1567810.2066463898441658No Hit
CTCCGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCG1551430.2044874114822167No Hit
GGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGC1547850.20401554685854284No Hit
GCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGC1515360.19973317769006135No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT1499500.19764273832372967No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG1498530.197514886735751No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCC1486300.19590290228113327No Hit
GGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCG1483350.19551407528676518No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC1481300.19524387347711955No Hit
CCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACCG1479430.1949973967044184No Hit
CGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGA1475370.1944622653155592No Hit
AGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTAC1436930.18939565187030133No Hit
GGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACCGAAGAAGC1413530.1863113970675169No Hit
CGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGAC1362730.17961566441873697No Hit
CCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGG1291810.17026799986260568No Hit
CCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGAC1282580.16905143269039627No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA1262820.16644695085693384No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA1262750.16643772445367763No Hit
CGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGG1253990.16528310598904553No Hit
CCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGA1241070.16358017555947393No Hit
GGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGT1213320.1599225656971975No Hit
GAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACCGAAG1160900.15301330771591715No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA1133640.14942028267643406No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG1123260.14805213887930146No Hit
GCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGG1116600.14717431251235513No Hit
CGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGG1105040.1456506379174753No Hit
GTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAA1103610.14546215567952736No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC1100230.14501665220801405No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG1072320.1413379534240092No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1069170.14092276527748052No Hit
CCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACC1055060.13906298599255365No Hit
GCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACCGAAGAA1051750.13862670892429654No Hit
GTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCC1045800.13784246464752015No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTCATCAATTCTTT1044190.1376302573726277No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG1042420.13739696117600683No Hit
CTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCG1041860.1373231499499573No Hit
GAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCC1029490.13569271268882724No Hit
CCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGC1018330.1342217603982685No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT1001080.131948111024421No Hit
CGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGAT998790.1316462758321827No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG994070.13102415264119371No Hit
GACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGG991870.13073417996742762No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA987060.13010019425796637No Hit
GCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGA976640.12872677823040168No Hit
CTCCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGA974920.12850007232182095No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGA965320.12723473701811452No Hit
CCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAG955570.12594963085028765No Hit
CCGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGA947080.12483059994107228No Hit
CGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACG934990.12323706829296699No Hit
GGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCC934200.1231329417419328No Hit
GGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGAC932770.12294445950398486No Hit
GGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCC927020.12218657637936903No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGC910310.11998410211635502No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGAC904700.11924467179825157No Hit
GGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTG897690.11832071341502425No Hit
GGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAA884330.11655978845069948No Hit
GTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGAT879120.11587308043691713No Hit
CCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCG877540.11566482733484876No Hit
CACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAA862590.11369433121084759No Hit
ACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAAC853030.11243426813757328No Hit
CCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCT838130.11047036230161222No Hit
GCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATC837060.11032933013755328No Hit
CCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCC820520.10814926285387574No Hit
CGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC813970.1072859351206177No Hit
CTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACA801410.10563045476493511No Hit
CCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCG801170.10559882138234243No Hit
CCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGA797300.10508873308803578No Hit
CTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAG795000.10478557983818945No Hit
GTCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCG787990.10386162145496214No Hit
CGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACCGA781080.10295084364781511No Hit
GGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTC776060.10228917872858528No Hit
GGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCC775850.10226149951881672No Hit
GGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGG772750.10185290166032816No Hit
CCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGAT764920.1008208625532426No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)