FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84646047
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG12743301.5054808170782035TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT7850340.92743137786458TruSeq Adapter, Index 13 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4667860.5514563485758526No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG1935660.22867695168328414No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC1600650.1890992027070089No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1487090.17568333699032632No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC1476300.1744086170970276No Hit
CTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCACGTG1455160.1719111584738269No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1273370.15043466826040913No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1015010.1199122742258714No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA924010.10916162452335193No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG913280.10789399297051638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)