FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007698986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007698986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82430157
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG8861571.0750398061233826TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT2168390.26305785150936933TruSeq Adapter, Index 19 (97% over 38bp)
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG1534600.18616972911988994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040440.12622079562459163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)