FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77041164
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG5767990.7486893630007979TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT3549430.4607186360787592TruSeq Adapter, Index 6 (97% over 36bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1148910.14912936673698232No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT911080.11825885704426792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)