FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90847864
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG5225480.5751901882910533TruSeq Adapter, Index 1 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2812280.30955928694151796No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG2119470.23329882582599853No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1910970.2103483687849832TruSeq Adapter, Index 1 (97% over 36bp)
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC1162170.12792485687940885No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC986920.10863436481016218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)