FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89218941
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG2280840.25564526707395013TruSeq Adapter, Index 1 (97% over 35bp)
CGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC1413690.15845177987485862No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1192620.13367340910267025TruSeq Adapter, Index 1 (97% over 36bp)
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGG1019890.11431317033902028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)