FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74120319
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC5305810.7158374480282526Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT2607160.3517470020602583Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC775820.10467035361787906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)