FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72584968
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC9271991.2773980970825807Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT1918920.2643687877633286Illumina Single End PCR Primer 1 (96% over 33bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA1317240.18147559147508338No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT926370.12762559873278445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)