FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83270356
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC4773570.5732616298650146Illumina Single End PCR Primer 1 (96% over 32bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1027470.12338964901266905No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT902700.10840592539318554Illumina Single End PCR Primer 1 (96% over 33bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA881340.10584078684616167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)