FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60557464
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG21395543.533097092705203TruSeq Adapter, Index 27 (97% over 38bp)
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG5346580.8828936429702539No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3885660.6416484019211901No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3460510.5714423576258081TruSeq Adapter, Index 27 (97% over 39bp)
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT1520170.25102933636719No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT1436070.23714170064981585No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT978030.16150445137530858No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC968010.15984982462277483No Hit
CCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCC959680.158474271643872No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGA957130.15805318399726911No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG946600.15631433971541475No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG928090.15325773879830898No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT923720.1525361101647189No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG915010.15109780686985175No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC908550.15003105149845772No Hit
CCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGT865400.1429055879882949No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC865090.1428543969410608No Hit
GTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTG840450.13878553434800375No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA839320.138598934724215No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTG832780.13751896875998637No Hit
CTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC818410.13514601602207119No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT806340.1331528678281508No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA788950.13028121521072944No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG775030.12798257205750888No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA772650.1275895569206795No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA771660.1274260758343513No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC770840.12729066725779667No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG755880.12482028639772628No Hit
AGTATGGCCCGGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCA755250.12471625297915381No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT753310.12439589610291474No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT748280.12356528007843923No Hit
CTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGC746920.123340700000251No Hit
AGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGC744900.12300713253117733No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG740570.12229210919400454No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA738810.12200147615164333No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC723640.11949641748538214No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGA716250.11827608897228589No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA712980.11773610599017158No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC699040.11543416018874238No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG693520.11452262928315492No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA686320.11333367592804085No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGG681170.11248324401431341No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA669120.11049339846860165No Hit
AGTATGGCCCGGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTG667720.11026221309399616No Hit
GATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAG661830.10928958319654865No Hit
CTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT650000.10733606678113206No Hit
GATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTCTGTG648640.10711148670294385No Hit
CTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCC643860.10632215378107643No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGAT640880.10583005919798756No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT626690.10348683029395023No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA622000.10271235928902174No Hit
GATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCCCCGC621270.102591812629406No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT616240.10176119660493049No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA605940.10006033277747561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)