FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74442442
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC5577420.749225824698228Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT996250.13382822664522478Illumina Single End PCR Primer 1 (96% over 32bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA943930.12679997789433076No Hit
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC925490.12432289633916094No Hit
GTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGG831610.1117118108511271No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA772620.10378756785007133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)