FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78576409
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC3727680.47440192895554695Illumina Single End PCR Primer 1 (96% over 33bp)
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC1078000.1371913038174091No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT883270.1124090565146595Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)