FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69892230
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG31017024.437835221454516TruSeq Adapter, Index 3 (97% over 35bp)
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC4344750.6216356238740701No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT3958500.5663719701031145TruSeq Adapter, Index 3 (97% over 36bp)
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC3396960.4860282752460467No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG1932300.2764685001465828No Hit
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGA1513180.21650189155504124No Hit
ACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTG1285650.18394748600810135No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1178120.1685623709531088No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG1133020.16210957927655192No Hit
GCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT1080700.15462376862206284No Hit
CCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCAC821280.11750662412688792No Hit
TACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCT734750.1051261349079862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)