FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82899094
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG10016601.2082882353334283TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT5946280.7172913132199008TruSeq Adapter, Index 6 (97% over 36bp)
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG3003160.36226692658426396No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2069160.24959983277983716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)