FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75556098
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC2397350.31729404554480833Illumina Single End PCR Primer 1 (96% over 33bp)
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT1278010.16914716797577345No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA1041360.1378260693134259No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT865710.11457844210006715No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT831150.11000435729224661Illumina Single End PCR Primer 1 (97% over 34bp)
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA801050.10602056236413904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)