FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75662636
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG3334930.44076312646574983TruSeq Adapter, Index 19 (97% over 37bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1028700.1359587842009628No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA979980.1295196746780009No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG876180.11580088222144416No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT858660.11348534037328543No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT844260.11158215529260705TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)