FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84842813
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC1780340.20983981283128839No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC1761560.20762630772272958Illumina Single End PCR Primer 1 (96% over 33bp)
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG1519610.17910886570910844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)