FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94809390
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG2870430.302757986313381TruSeq Adapter, Index 27 (97% over 38bp)
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG2231910.2354102267718419No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1933060.20388908735727548No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1032510.10890376997468287TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)