FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88781586
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG5747060.6473256740423627TruSeq Adapter, Index 7 (97% over 34bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2529770.2849430961956458No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC2483390.2797190399369527No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC1459310.16437079643970315No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1443390.16257763180756876TruSeq Adapter, Index 7 (97% over 35bp)
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG1089720.12274166852572334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)