FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87441806
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG3950110.4517415845688274TruSeq Adapter, Index 6 (97% over 35bp)
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1046710.1197036117941114No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA956350.10936988195326157No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT933480.10675442819650821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)