FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80855118
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3653290.4518316329709642No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG3524150.4358598549073913TruSeq Adapter, Index 13 (97% over 37bp)
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC1574700.19475576054443455No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC975330.12062687237683581No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA889360.10999427395554601No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT886520.10964302841039697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)