FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79244900
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC11377311.4357151059563455Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT1707780.21550661304386784Illumina Single End PCR Primer 1 (97% over 34bp)
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC1413100.17832062378777688No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1211430.15287166745115457No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC933320.11777666449197362No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC826550.10430324222757553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)