FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90733194
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG5851160.6448753473838913TruSeq Adapter, Index 3 (97% over 35bp)
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC2535440.2794390771694866No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC1789600.1972376283810752No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1732850.19098302656467708TruSeq Adapter, Index 3 (97% over 36bp)
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTT1196610.13188227452898882No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCC1010000.11131538034470603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)