FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90733194
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC5875770.6475876954138746Illumina Single End PCR Primer 1 (96% over 33bp)
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC1934510.21320863012934385No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT1744760.1922956663467617Illumina Single End PCR Primer 1 (97% over 34bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC977300.10771140713948635No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGC960750.1058873778873033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)