FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73951797
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG4116340.556624743006583TruSeq Adapter, Index 2 (97% over 36bp)
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG1206830.1631914367138367No Hit
CGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC1116120.15092533856885182No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1020130.1379452618304867TruSeq Adapter, Index 2 (97% over 37bp)
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGG1007290.13620899570567568No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC936210.12659732933873127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)