FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73951797
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC4078750.5515417022253022Illumina Single End PCR Primer 1 (96% over 31bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1516950.20512686121744952No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1126420.15231813771881703No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC1068460.14448059997784773No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT1012490.13691215644158045Illumina Single End PCR Primer 1 (96% over 32bp)
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG887130.11996057377753781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)