FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93615811
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG11474541.2257053458629974TruSeq Adapter, Index 3 (97% over 35bp)
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC3858400.41215260101736445No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC2919670.31187787285205487No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1604820.1714261707351977TruSeq Adapter, Index 3 (97% over 36bp)
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG1467020.15670643498457756No Hit
ACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTG1351760.14439441217894272No Hit
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGA1308000.13971998811183722No Hit
GCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT1051610.11233252041153603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)